Hatta S, Mishima Y, Ichihashi M, Ito S
Department of Dermatology, Kobe University of Medicine, Japan.
J Invest Dermatol. 1988 Aug;91(2):181-4. doi: 10.1111/1523-1747.ep12464454.
We have found substantial amounts (6.6-143 and 0.5-13 micrograms/mg. protein, respectively) of 5,6-dihydroxyindole (5,6-DHI) and 5,6-dihydroxyindole-2-carboxylic acid (5,6-DHI2C), which are key intermediate monomers for the formation of the eumelanin polymer, within coated vesicle fraction of pigment cells. In addition, the amounts of these eumelanin monomers have been found to decrease along with the process of eumelanin polymer formation from coated vesicles to premelanosomes and finally to melanosomes among melanogenic subcellular compartments. Our present findings seem to indicate that coated vesicles transfer not only highly glycosylated T1-tyrosinase but also eumelanin monomers into premelanosomes.
我们在色素细胞的被膜小泡组分中发现了大量（分别为6.6 - 143和0.5 - 13微克/毫克蛋白质）的5,6 - 二羟基吲哚（5,6 - DHI）和5,6 - 二羟基吲哚 - 2 - 羧酸（5,6 - DHI2C），它们是真黑素聚合物形成的关键中间单体。此外，已发现这些真黑素单体的量随着真黑素聚合物从被膜小泡到前黑素体并最终到黑素体的形成过程在黑素生成亚细胞区室中减少。我们目前的研究结果似乎表明，被膜小泡不仅将高度糖基化的T1 - 酪氨酸酶，而且还将真黑素单体转运到前黑素体中。
