School of Studies in Biotechnology, Jiwaji University, Gwalior, India.
J Food Biochem. 2019 Feb;43(2):e12712. doi: 10.1111/jfbc.12712. Epub 2018 Nov 14.
A wild chickpea lectin (WCL) from the seeds of Cicer reticulatum L. was chromato-purified using DEAE-Cellulose and SP-Sephadex ion exchange chromatography. WCL was thermostable upto 60°C with broad pH optima (pH 5-9) and various divalent metal ions did not influence its activity. WCL demonstrated DNA protection in a dose-dependent manner. The lectin exerted antifungal activity against diverse fungal pathogens. WCL augmented the mitogenic response of mouse spleen cells at 10 µg/ml concentration and showed an inhibition of HIV-1 reverse transcriptase at IC of 200 µM. Against human cancer cell lines, lectin demonstrated anticancer potential. The cell viability assay in HepG2, Ishikawa, MCF-7 and MDA-MB-231 cell line demonstrated IC values of 61.8, 54.4, 37.5 and 44.2 µg/ml respectively. PRACTICAL APPLICATIONS: WCL exhibited distinct medicinal properties vis-à-vis antiproliferative, mitogenic, antifungal/bacterial and HIV-1 reverse transcriptase inhibiting activities. The potential of WCL can be a subject of exploration from a pharmacological standpoint.
从 Cicer reticulatum L. 种子中纯化出一种野生鹰嘴豆凝集素（WCL），采用 DEAE-纤维素和 SP-Sephadex 离子交换层析进行层析纯化。WCL 在 60°C 以下具有热稳定性，最适 pH 范围较宽（pH5-9），并且各种二价金属离子不会影响其活性。WCL 以剂量依赖的方式显示出 DNA 保护作用。该凝集素对多种真菌病原体具有抗真菌活性。WCL 在 10μg/ml 浓度下增强了小鼠脾细胞的有丝分裂反应，并在 IC 为 200μM 时显示出抑制 HIV-1 逆转录酶的活性。针对人癌细胞系，凝集素显示出抗癌潜力。在 HepG2、Ishikawa、MCF-7 和 MDA-MB-231 细胞系中的细胞活力测定表明，IC 值分别为 61.8、54.4、37.5 和 44.2μg/ml。实际应用：WCL 表现出明显的药用特性，具有抗增殖、有丝分裂原、抗真菌/细菌和 HIV-1 逆转录酶抑制活性。从药理学角度来看，WCL 的潜力可以作为探索的主题。
