Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
Department of Anesthesiology, China Medical University Hospital, Taichung, Taiwan.
J Food Biochem. 2019 Jul;43(7):e12902. doi: 10.1111/jfbc.12902. Epub 2019 May 16.
Casticin, a polymethoxyflavone derived from natural plants, has biological activities including induction of cell apoptosis. In this study, we showed the beneficial effects of casticin on the inhibition of prostate cancer cell metastasis. Casticin reduced total viable cell number, thus, we selected low doses of casticin for following experiments. Casticin decreased cell mobility, suppressed cell migration and invasion, and reduced cell gelatinolytic activities of MMP-2/-9. Furthermore, casticin inhibited the protein levels of AKT, GSK3 αβ, Snail, and MMPs (MMP-2, -9, -13, and -7) at 24 and 48 hr treatment. Casticin diminished the expressions of NF-κB p65, GRB2, SOS-1, MEK, p-ERK1/2, and p-JNK1/2 at 48 hr treatment only. However, casticin reduced the level of E-cadherin at 24 hr treatment but elevated at 48 hr. The novel findings suggest that casticin may represent a new and promising therapeutic agent for the metastatic prostate cancer. PRACTICAL APPLICATIONS: Casticin derived from natural plants had been used for Chinese medicine in Chinese population for thousands of years. In the present study, casticin attenuated metastatic effects, including decreasing viable cell number, inhibiting the migration, invasion, and adhesion, and reducing matrix metalloproteinases activity on human prostate DU 145 cancer cells. In addition, the results also provided possible pathways involved in casticin anti-metastasis mechanism. We conclude that casticin may be an aptitude anticancer agent or adjuvant for the metastatic prostate cancer in the future.
金合欢素是一种源自天然植物的多甲氧基黄酮，具有诱导细胞凋亡等生物学活性。在本研究中，我们显示了金合欢素抑制前列腺癌细胞转移的有益作用。金合欢素降低了总活细胞数量，因此，我们选择低剂量的金合欢素进行后续实验。金合欢素降低了细胞迁移率，抑制了细胞迁移和侵袭，并降低了 MMP-2/-9 的细胞明胶酶活性。此外，金合欢素在 24 和 48 小时处理时抑制了 AKT、GSK3αβ、Snail 和 MMPs（MMP-2、-9、-13 和 -7）的蛋白水平。金合欢素在 48 小时处理时仅降低了 NF-κB p65、GRB2、SOS-1、MEK、p-ERK1/2 和 p-JNK1/2 的表达水平，但在 24 小时处理时降低了 E-钙粘蛋白的水平，而在 48 小时处理时升高了 E-钙粘蛋白的水平。这些新发现表明，金合欢素可能代表一种新的、有前途的治疗转移性前列腺癌的药物。实际应用：金合欢素源自天然植物，已被中国人用作中药数千年。在本研究中，金合欢素减弱了转移性作用，包括降低活细胞数量、抑制迁移、侵袭和黏附，以及降低人前列腺 DU145 癌细胞基质金属蛋白酶的活性。此外，结果还提供了金合欢素抗转移机制中可能涉及的途径。我们得出结论，金合欢素可能是未来治疗转移性前列腺癌的一种有前途的抗癌药物或辅助药物。
