Wang Yuesong, Ding Haichang, Wang Shuai, Fan Congbin, Tu Yayi, Liu Gang, Pu Shouzhi
Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, China.
Luminescence. 2019 Dec;34(8):911-917. doi: 10.1002/bio.3690. Epub 2019 Jul 28.
A new ratiometric probe composed of a dansyl-rhodamine dyad for the detection of Hg via fluorescence resonance energy transfer was designed and synthesized. Rhodamine, dansyl chloride, and hydrazide were selected as the acceptor, donor, and reaction site, respectively. It displayed high selectivity and sensitivity to Hg with obvious colour change and fluorescence change due to Hg -assisted hydrolysis of rhodamine hydrazide. A good linear relationship ranging from 0 to 16 μM and 0-28 μM for the Hg concentration was found based on absorbance and fluorescence assay, respectively. Detection limits of absorbance and fluorescence for Hg were calculated to be 1.22 μM and 9.10 μM, respectively.
设计并合成了一种由丹磺酰罗丹明二元体组成的新型比率探针，用于通过荧光共振能量转移检测汞。分别选择罗丹明、丹磺酰氯和酰肼作为受体、供体和反应位点。由于罗丹明酰肼的汞辅助水解，它对汞表现出高选择性和敏感性，伴有明显的颜色变化和荧光变化。基于吸光度和荧光测定，分别发现汞浓度在0至16μM和0至28μM范围内具有良好的线性关系。汞的吸光度和荧光检测限分别计算为1.22μM和9.10μM。
