用于铁死亡中内源性 Fe 比色跟踪的可逆 FRET 荧光探针。
Reversible FRET Fluorescent Probe for Ratiometric Tracking of Endogenous Fe in Ferroptosis.
机构信息
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
出版信息
Inorg Chem. 2020 Aug 3;59(15):10920-10927. doi: 10.1021/acs.inorgchem.0c01412. Epub 2020 Jul 12.
A reversible fluorescent probe is devised by linking spirolactam rhodamine and dansylamide through an Fe ionophore -hydroxyethyldiethylenetriamine, where Fe chelation generating rhodamine B can switch intramolecular fluorescence resonance energy transfer for ratiometric Fe sensing. Probe exhibits an Fe-specific emission shift from 483 to 576 nm, and this ratiometric response has been successfully applied to monitor labile Fe fluctuations in cells undergoing ferroptosis.
设计了一种通过 Fe 离子载体 - 羟乙基二乙三胺将螺环缩酮罗丹明和丹磺酰基酰胺连接起来的可逆荧光探针，其中 Fe 螯合生成罗丹明 B 可以切换分子内荧光共振能量转移，实现比率型 Fe 传感。探针表现出 Fe 特异性发射位移，从 483nm 到 576nm，这种比率响应已成功应用于监测发生铁死亡的细胞中不稳定 Fe 的波动。
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