Li Qianqian, Liu Juanjuan, Shao Jing, Da Wenyue, Shi Gaoxiang, Wang Tianming, Wu Daqiang, Wang Changzhong
Laboratory of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine (College of Life Science), Anhui University of Chinese Medicine, Hefei, China.
Institute of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China.
Front Microbiol. 2019 Jul 11;10:1600. doi: 10.3389/fmicb.2019.01600. eCollection 2019.
and are two commonly seen opportunistic fungi in clinical settings and usually co-isolated from the population inflicted with denture stomatitis and oropharyngeal candidiasis. Although and mixed biofilm is deemed to possess enhanced virulence compared with their individual counterparts (especially single biofilm), the relevant descriptions and experimental evidence on the relationship of virulence with their individual cell number in mixed biofilms are contradictory and insufficient. In this study, two standard isolate and eight ones were used to test the cell quantities in their 24- and 48-h single and mixed biofilms. A series of virulence factors including antifungal resistance to caspofungin, secreted aspartic proteinase (SAP) and phospholipase (PL) levels, efflux pump function and β-glucan exposure were evaluated. Through this study, the declines of individual cell counting were observed in the 24- and 48-h mixed biofilms compared with their single counterparts. However, the antifungal resistance to caspofungin, the SAP and phospholipase levels, the rhodamine 6G efflux and the efflux-related gene expressions were increased significantly or kept unchanged accompanying with reduced β-glucan exposure in the mixed biofilms by comparison with the single counterparts. These results reveal that there is a competitive interaction between and strains in their co-culture without at the expense of the mixed biofilm virulence. This study presents a deep insight into the interaction between and and provides new clues to combat against fungal infections caused by mixed biofilms.
[菌名1]和[菌名2]是临床环境中常见的两种机会性真菌，通常从患有义齿性口炎和口腔念珠菌病的人群中共同分离得到。尽管[菌名1]和[菌名2]的混合生物膜被认为与其单独的生物膜相比具有更强的毒力（尤其是[菌名1]单一生物膜），但关于混合生物膜中毒力与其单个细胞数量关系的相关描述和实验证据相互矛盾且不足。在本研究中，使用了两种标准的[菌名1]分离株和八种[菌名2]分离株来检测它们在24小时和48小时单一及混合生物膜中的细胞数量。评估了一系列毒力因子，包括对卡泊芬净的抗真菌耐药性、分泌天冬氨酸蛋白酶（SAP）和磷脂酶（PL）水平、外排泵功能以及β-葡聚糖暴露情况。通过本研究，观察到与单一生物膜相比，在24小时和48小时的[菌名1]和[菌名2]混合生物膜中单个细胞计数下降。然而，与单一生物膜相比，混合生物膜中对卡泊芬净的抗真菌耐药性、SAP和磷脂酶水平、罗丹明6G外排以及外排相关基因表达显著增加或保持不变，同时β-葡聚糖暴露减少。这些结果表明，[菌名1]和[菌名2]菌株在共培养中存在竞争性相互作用，而不会以混合生物膜毒力为代价。本研究深入洞察了[菌名1]和[菌名2]之间的相互作用，并为对抗由[菌名1]和[菌名2]混合生物膜引起的真菌感染提供了新线索。
