Department of General Surgery, Hainan General Hospital, Haikou 570311, China.
Department of Anesthesiology, Hainan General Hospital, Haikou 570311, China.
Can J Gastroenterol Hepatol. 2019 Jul 1;2019:8348967. doi: 10.1155/2019/8348967. eCollection 2019.
HMGCS2 is the rate-limiting enzyme of ketogenesis, which is vital for tumor initiation or metastasis. The aim of this study is to determine the relationship between HMGCS2 and tumor angiogenesis.
The study consisted of 100 cases with colorectal cancer and healthy control, the expression of HMGCS2 and the microvessel density (MVD) (marker: CD31) were analyzed by immunohistochemistry and tube formation, and the centration of -hydroxybutyrate in serum was assessed by biochemical analysis.
The results showed that HMGCS2 expression is significantly reduced in colorectal cancer compared with healthy control, which is inversely correlated with MVD in colorectal cancer by IHC analysis. What is more, knockdown HMGCS2 expression in HT-29 cells significantly contributed endothelial cell tube formation.
These findings implying HMGCS2 may have a negative regulation of tumor angiogenesis and provide an approach to inhibit tumor angiogenesis.
HMGCS2 是酮体生成的限速酶，对肿瘤的发生或转移至关重要。本研究旨在确定 HMGCS2 与肿瘤血管生成之间的关系。
本研究共纳入 100 例结直肠癌患者和健康对照者，采用免疫组织化学和管形成实验分析 HMGCS2 和微血管密度（标志物：CD31）的表达，并通过生化分析评估血清中β-羟丁酸的浓度。
结果显示，与健康对照组相比，结直肠癌中 HMGCS2 的表达明显降低，通过 IHC 分析，其与结直肠癌中的 MVD 呈负相关。此外，在 HT-29 细胞中敲低 HMGCS2 表达显著促进内皮细胞管形成。
这些发现表明 HMGCS2 可能对肿瘤血管生成具有负调控作用，并为抑制肿瘤血管生成提供了一种方法。
