Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, China.
Biomed Res Int. 2019 Jul 2;2019:2932585. doi: 10.1155/2019/2932585. eCollection 2019.
Rubredoxins are a class of iron-containing proteins that play an important role in the reduction of superoxide in some anaerobic bacteria and also act as electron carriers in many biochemical processes. Unlike the more widely studied about rubredoxin proteins in anaerobic bacteria, very few researches about the function of rubredoxins have been proceeded in plants. Previous studies indicated that rubredoxins in may play a critical role in responding to oxidative stress. In order to identify more rubredoxins in plants that maybe have similar functions as the rubredoxin-like protein of , we identified and analyzed plant rubredoxin proteins using bioinformatics-based methods. Totally, 66 candidate rubredoxin proteins were identified based on public databases, exhibiting lengths of 187-360 amino acids with molecular weights of 19.856-37.117 kDa. The results of subcellular localization showed that these candidate rubredoxins were localized to the chloroplast, which might be consistent with the fact that rubredoxins were predominantly expressed in leaves. Analyses of conserved motifs indicated that these candidate rubredoxins contained rubredoxin and PDZ domains. The expression patterns of rubredoxins in glycophyte and halophytic plant under salt/drought stress revealed that rubredoxin is one of the important stress response proteins. Finally, the coexpression network of rubredoxin in under abiotic was extracted from ATTED-II to explore the function and regulation relationship of rubredoxin in . Our results showed that putative rubredoxin proteins containing PDZ and rubredoxin domains, localized to the chloroplast, may act with other proteins in chloroplast to responses to abiotic stress in higher plants. These findings might provide value inference to promote the development of plant tolerance to some abiotic stresses and other economically important crops.
类菌红素是一类含铁的蛋白质，在一些厌氧菌中超氧化物的还原中起着重要作用，同时在许多生化过程中也作为电子载体。与在厌氧菌中研究得更为广泛的类菌红素蛋白不同，关于植物类菌红素的功能研究很少。先前的研究表明， 中的类菌红素可能在应对氧化应激中发挥关键作用。为了鉴定植物中可能具有与类菌红素样蛋白相似功能的更多类菌红素，我们使用基于生物信息学的方法鉴定和分析了植物类菌红素蛋白。总共根据公共数据库鉴定了 66 种候选类菌红素蛋白，其长度为 187-360 个氨基酸，分子量为 19.856-37.117 kDa。亚细胞定位的结果表明这些候选类菌红素定位于叶绿体，这可能与类菌红素主要在叶片中表达的事实一致。保守基序分析表明，这些候选类菌红素含有类菌红素和 PDZ 结构域。盐/干旱胁迫下糖生植物和盐生植物中类菌红素的表达模式表明，类菌红素是重要的应激响应蛋白之一。最后，从 ATTED-II 中提取了 中类菌红素在非生物胁迫下的共表达网络，以探索类菌红素在 中的功能和调控关系。我们的结果表明，含有 PDZ 和类菌红素结构域、定位于叶绿体的假定类菌红素蛋白可能与叶绿体中的其他蛋白一起作用，以响应高等植物中的非生物胁迫。这些发现可能为促进植物对一些非生物胁迫和其他经济上重要作物的耐受性的发展提供有价值的推断。
