Centre for Computational Biology, Peter the Great St. Petersburg Polytechnic University, 195251 St. Petersburg, Russia.
Mathematical Biology & Bioinformatics Laboratory, Peter the Great St. Petersburg Polytechnic University, 195251 St. Petersburg, Russia.
Int J Mol Sci. 2021 Nov 17;22(22):12383. doi: 10.3390/ijms222212383.
Modern flax cultivars are susceptible to many diseases; arguably, the most economically damaging of these is the Fusarium wilt fungal disease. Over the past decades international flax breeding initiatives resulted in the development of resistant cultivars. However, much remains to be learned about the mechanisms of resistance to Fusarium infection in flax. As a first step to uncover the genetic factors associated with resistance to Fusarium wilt disease, we performed a genome-wide association study (GWAS) using 297 accessions from the collection of the Federal Research Centre of the Bast Fiber Crops, Torzhok, Russia. These genotypes were infected with a highly pathogenic f.sp. MI39 strain; the wilt symptoms were documented in the course of three successive years. Six different single-locus models implemented in GAPIT3 R package were applied to a selected subset of 72,526 SNPs. A total of 15 QTNs (Quantitative Trait Nucleotides) were detected during at least two years of observation, while eight QTNs were found during all three years of the experiment. Of these, ten QTNs occupied a region of 640 Kb at the start of chromosome 1, while the remaining QTNs mapped to chromosomes 8, 11 and 13. All stable QTNs demonstrate a statistically significant allelic effect across 3 years of the experiment. Importantly, several QTNs spanned regions that harbored genes involved in the pathogen recognition and plant immunity response, including the KIP1-like protein () and NBS-LRR protein (). Our results provide novel insights into the genetic architecture of flax resistance to Fusarium wilt and pinpoint potential candidate genes for further in-depth studies.
现代亚麻品种易患多种疾病；其中最具经济破坏性的当属镰刀菌枯萎病真菌病。在过去几十年中，国际亚麻育种计划促成了抗品种的发展。然而，对于亚麻对镰刀菌感染的抗性机制，仍有许多需要了解。作为揭示与抗镰刀菌枯萎病相关的遗传因素的第一步，我们使用来自俄罗斯托罗克联邦研究中心的巴斯特纤维作物收藏的 297 个品系进行了全基因组关联研究（GWAS）。这些基因型被高度致病的 f.sp. MI39 菌株感染；在连续三年的时间里记录了萎蔫症状。在 GAPIT3 R 包中实施的六种不同的单基因座模型被应用于从 72,526 个 SNP 中选择的一个子集。在至少两年的观察期间，共检测到 15 个 QTN（数量性状核苷酸），而在实验的三年中发现了 8 个 QTN。其中，十个 QTN 占据了染色体 1 起始处 640 Kb 的区域，而其余 QTN 则映射到染色体 8、11 和 13。所有稳定的 QTN 在实验的 3 年内均显示出具有统计学意义的等位基因效应。重要的是，几个 QTN 跨越了包含参与病原体识别和植物免疫反应的基因的区域，包括 KIP1 样蛋白（）和 NBS-LRR 蛋白（）。我们的研究结果为亚麻对镰刀菌枯萎病的抗性遗传结构提供了新的见解，并确定了潜在的候选基因，以进行进一步的深入研究。
