Krahmer Natalie, Mann Matthias
Institute for Diabetes and Obesity, Helmholtz Zentrum München, Munich-Neuherberg, Germany.
Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
Contact (Thousand Oaks). 2019 Jan 1;2:2515256419859186. doi: 10.1177/2515256419859186. Epub 2019 Jul 4.
Lipid droplets (LDs), important organelles for energy storage and involved in the development of metabolic disorders, are extremely dynamic and interact with many other cellular compartments to orchestrate lipid metabolism. Little is known about how these organelle contacts are changed according to cellular needs and functions under different metabolic and pathological conditions and which proteins regulate this. Here, we summarize recent exciting discoveries about the reorganization of organelle contacts in steatotic liver, including the identification of novel LD contact site proteins in cell lines and in animals. We also discuss state of the art proteomics workflows that enable the characterization of LD-organelle contacts and tethering proteins and give an outlook how this can inform obesity research.
脂滴（LDs）是能量储存的重要细胞器，参与代谢紊乱的发展，极具动态性，并与许多其他细胞区室相互作用以协调脂质代谢。关于这些细胞器接触如何根据不同代谢和病理条件下的细胞需求和功能而改变，以及哪些蛋白质调节这一过程，我们知之甚少。在这里，我们总结了关于脂肪变性肝脏中细胞器接触重组的最新令人兴奋的发现，包括在细胞系和动物中鉴定新的脂滴接触位点蛋白。我们还讨论了能够表征脂滴 - 细胞器接触和连接蛋白的蛋白质组学工作流程，并展望这如何为肥胖研究提供信息。
