State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Food Funct. 2019 Aug 1;10(8):4998-5007. doi: 10.1039/c8fo02062k. Epub 2019 Jul 29.
Grifola Frondosa, the king of mushrooms, is one of the most valued traditional medicines and has been used as a health food for a long time in China, Japan, and other Asian countries. The present study was designed to evaluate the immune-modulating effects of water-soluble polysaccharides from the Grifola Frondosa fruiting body (GFP) by using mouse peritoneal macrophage and cytoxan (CTX) induced immunosuppression models. Compared with CTX-induced immunosuppressive mice, the spleen and thymus indexes in mice with GFP orally administrated were significantly increased, body weight loss was alleviated, and the natural killer (NK) cytotoxicity and the proliferative activities of lymphocytes were elevated. Furthermore, levels of interleukin-2 (IL-2), interferon-6 (IL-6) and tumor necrosis factor-α (TNF-α) were notably reduced by CTX, while GFP abolished these effects. GFP also effectively increased total antioxidant capacity and superoxidase dismutase, catalase and glutathione peroxidase activities, and inhibited an increase in the malondialdehyde level. Histopathological analysis of spleens revealed the protective effect of GFP against CTX-induced immunosuppression. Western blotting results showed that GFP possessed immunomodulatory activity by up-regulating transcription factors (p-JAK2/JAK2, p-STAT3/STAT3 and SOCS3) in JAK2/STAT3/SOCS signaling pathways. This study suggested that GFP may provide an alternative strategy for lessening chemotherapy-induced immunosuppression.
灰树花，俗称“舞菇”，是一种珍贵的药用真菌，在中国、日本和其他亚洲国家，它已经作为一种健康食品被长期使用。本研究旨在通过利用小鼠腹腔巨噬细胞和环磷酰胺（CTX）诱导的免疫抑制模型，评估灰树花子实体水溶性多糖（GFP）的免疫调节作用。与 CTX 诱导的免疫抑制小鼠相比，口服 GFP 的小鼠脾脏和胸腺指数显著增加，体重减轻得到缓解，自然杀伤（NK）细胞毒性和淋巴细胞的增殖活性提高。此外，CTX 显著降低了白细胞介素 2（IL-2）、干扰素 6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平，而 GFP 则消除了这些作用。GFP 还能有效增加总抗氧化能力和超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性，并抑制丙二醛水平的升高。对脾脏的组织病理学分析显示，GFP 对 CTX 诱导的免疫抑制具有保护作用。Western blot 结果表明，GFP 通过上调 JAK2/STAT3/SOCS 信号通路中的转录因子（p-JAK2/JAK2、p-STAT3/STAT3 和 SOCS3）发挥免疫调节作用。本研究表明，GFP 可能为减轻化疗引起的免疫抑制提供一种替代策略。
