Zan Ke, Zhao Lei, Guo Li-Nong, Zheng Jian, Ma Shuang-Cheng
National Institutes for Food and Drug Control Beijing 100050,China.
Siping Institute for Food and Drug Control Siping 136000,China.
Zhongguo Zhong Yao Za Zhi. 2019 May;44(10):1974-1977. doi: 10.19540/j.cnki.cjcmm.20181101.004.
The aim of this paper was to compare the influence of freeze-drying and sun-drying on six main nucleosides and nucleobases of Cordyceps sinensis by HPLC. Hypoxanthine,xanthine,uridine,inosine,guanosine and adenosine were reference substances. HPLC analysis was performed on a GL Inertsustain AQ-C_(18) column( 4. 6 mm×250 mm,5 μm),with mobile phase consisting of water( A)-acetonitrile( B) at the flow rate of 1. 0 mL·min(-1)( 0-10 min,0-1% B; 10-65 min,1%-3% B). The detection wavelength was 260 nm,the column temperature was controlled at 30 ℃,and the injection volume was 5 μL. The linear ranges of hypoxanthine,xanthine,uridine,inosine,guanosine and adenosine were 1. 025-20. 50( r = 0. 999 8),0. 545-10. 90( r = 0. 999 9),4. 051-81. 02( r = 0. 999 8),4. 044-80. 88( r= 0. 999 9),2. 075-41. 50( r= 0. 999 9),4. 032-80. 64( r = 0. 999 9) mg·L(-1),respectively. The average recoveries of them( n = 6)were as follows: 102. 3%( RSD 2. 1%),101. 1%( RSD 2. 4%),97. 80%( RSD 1. 7%),101. 8%( RSD 1. 8%),98. 90%( RSD2. 0%) and 98. 10%( RSD 1. 4%),respectively. Each sample was processed by freeze-drying and sun-drying so as to compare the difference between the two drying methods. The contents of six index ingredients were significantly different between freeze-drying and sun-drying sample of C. sinensis. The total contents of six index ingredients in sun-drying sample were higher than that in the corresponding freeze-drying sample. This research results provide the scientific basis for the drying methods and quality evaluation of C. sinensis.
本文旨在通过高效液相色谱法比较冻干和晒干对冬虫夏草六种主要核苷和碱基的影响。以次黄嘌呤、黄嘌呤、尿苷、肌苷、鸟苷和腺苷为对照品。采用GL Inertsustain AQ-C₁₈柱（4.6 mm×250 mm，5 μm）进行高效液相色谱分析，流动相为水（A）-乙腈（B），流速为1.0 mL·min⁻¹（0 - 10 min，0 - 1%B；10 - 65 min，1% - 3%B）。检测波长为260 nm，柱温控制在30℃，进样量为5 μL。次黄嘌呤、黄嘌呤、尿苷、肌苷、鸟苷和腺苷的线性范围分别为1.025 - 20.50（r = 0.999 8）、0.545 - 10.90（r = 0.999 9）、4.051 - 81.02（r = 0.999 8）、4.044 - 80.88（r = 0.999 9）、2.075 - 41.50（r = 0.999 9）、4.032 - 80.64（r = 0.999 9）mg·L⁻¹。它们的平均回收率（n = 6）分别如下：102.3%（相对标准偏差2.1%）、101.1%（相对标准偏差2.4%）、97.80%（相对标准偏差1.7%）、101.8%（相对标准偏差1.8%）、98.90%（相对标准偏差2.0%）和98.10%（相对标准偏差1.4%）。对每个样品分别进行冻干和晒干处理，以比较两种干燥方法之间的差异。冬虫夏草冻干样品和晒干样品中六种指标成分的含量存在显著差异。晒干样品中六种指标成分的总含量高于相应的冻干样品。本研究结果为冬虫夏草的干燥方法和质量评价提供了科学依据。
