Li Hong-Yan, Liu Jing-Ling, Jin Wei-Bo, Liang Zong-Suo
College of Life Science,Northwest Agriculture and Forestry University Yangling 712100,China.
College of Life Science,Zhejiang Sci-Tech University Hangzhou 310018,China.
Zhongguo Zhong Yao Za Zhi. 2019 May;44(10):2038-2045. doi: 10.19540/j.cnki.cjcmm.20190301.009.
The family of flavonoid 3-O-glucosyltransferase catalyzes the modification of anthocyanin from unstable-structure to stable-structure. In this study,based on homology cloning and transcriptome library,we isolated the full-length c DNA of UDP-glucose: flavonoid 3-O-glucosyltransferase( named SmUF3GT) from the flower tissues of S. miltiorrhiza. This gene was consisted of 1 353 bp open reading frames( ORF) encoding 450 amino acids. And the SmUF3GT protein was performed for the bioinformatic analysis. Our results showed that the protein was preliminary localized in the Golgi and peroxisome of cytosol,as well as plasma membrane and cell nuclear.QRT-PCR analyses indicated that SmUF3GT expressed differently in all tissues and organs but roots of S. miltiorrhiza and S. miltiorrhiza f.alba. During floral development,the expression of SmUF3GT showed a trend of rising fist and then down in purple-flower Danshen,whereas decreasing sharply fist and then slowly in white-flower Danshen. The present study provides basic information for further research on the network of synthesis and accumulation of flavonoids in S.miltiorrhiza.
类黄酮3-O-葡萄糖基转移酶家族催化花青素从不稳定结构向稳定结构的修饰。在本研究中，基于同源克隆和转录组文库，我们从丹参花组织中分离出UDP-葡萄糖：类黄酮3-O-葡萄糖基转移酶（命名为SmUF3GT）的全长cDNA。该基因由1353bp的开放阅读框（ORF）组成，编码450个氨基酸。并对SmUF3GT蛋白进行了生物信息学分析。我们的结果表明，该蛋白初步定位于细胞质的高尔基体和过氧化物酶体，以及质膜和细胞核。QRT-PCR分析表明，SmUF3GT在丹参和白花丹参除根以外的所有组织和器官中表达不同。在花发育过程中，SmUF3GT在紫花丹参中的表达呈现先上升后下降的趋势，而在白花丹参中则先急剧下降后缓慢下降。本研究为进一步研究丹参中黄酮类化合物的合成与积累网络提供了基础信息。
