下调可以增强骨肉瘤和的放射敏感性。

Downregulation of can enhance the radiosensitivity of osteosarcoma and .

机构信息

Department of Orthopaedics, Jinan Central Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences, No. 105, Jiefang Road, Jinan, Shandong 250013, China.

Department of Orthopaedics, Jinan Central Hospital Affiliated to Shandong University, No. 105, Jiefang Road, Jinan, Shandong 250013, China.

出版信息

Epigenomics. 2019 Aug;11(11):1283-1305. doi: 10.2217/epi-2019-0125. Epub 2019 Jul 29.

DOI:10.2217/epi-2019-0125

PMID:31355678

Abstract

To investigate the effect of gene on radiotherapy for osteosarcoma. Gene Expression Omnibus database, RT-qPCR and immunohistochemical analysis were performed. Cell proliferation and cell cycle experiments were conducted after knockdown of . Cell scratch, reactive oxygen species production and apoptosis experiments were conducted after the combination of radiotherapy and silencing. Then the xenograft mode was further conducted. was highly expressed in human osteosarcoma. Suppression of inhibited osteosarcoma cell proliferation and induced cell cycle arrest at the G2/M phase. Downregulation of combined with radiation can substantially inhibit clonal formation and migration, and promote apoptosis of osteosarcoma cells and . downregulation can enhance the radiosensitivity of osteosarcoma and .

摘要

探讨基因对骨肉瘤放疗的影响。利用基因表达综合数据库、实时荧光定量聚合酶链式反应和免疫组织化学分析进行研究。敲低基因后进行细胞增殖和细胞周期实验。在放疗和基因沉默联合作用后进行细胞划痕、活性氧产生和细胞凋亡实验。然后进一步进行异种移植模型。基因在人骨肉瘤中高表达。抑制基因表达可抑制骨肉瘤细胞增殖，并诱导细胞周期停滞在 G2/M 期。下调基因与放疗联合应用可显著抑制克隆形成和迁移，并促进骨肉瘤细胞凋亡。下调基因可增强骨肉瘤细胞和的放射敏感性。