Department of Nuclear Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200433, PR China.
Department of Emergency & Trauma Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, PR China.
Nanomedicine (Lond). 2019 Aug;14(15):1935-1944. doi: 10.2217/nnm-2019-0062. Epub 2019 Jul 29.
The diagnosis of vulnerable atherosclerotic plaques remains challenging. This study labeled ultrasmall superparamagnetic iron oxide with rhodamine (USPIO-R) and evaluated USPIO-R for imaging atherosclerotic plaques. Apolipoprotein E-deficient mice were fed a high-fat diet and underwent MRI before and after an intravenous injection of USPIO-R. Subsequently, an aortic specimen from the mice was removed and sliced for fluorescence imaging and Prussian blue and immunofluorescent staining. T signal loss appeared and persisted in the aortic plaque postinjection, and spontaneous fluorescence from the plaque was observed. The accumulated mechanism of USPIO-R by plaque was the macrophage internalization by Prussian blue and immunofluorescence. USPIO-R is a promising dual-modality probe for diagnosing and monitoring vulnerable atherosclerotic plaques.
易损动脉粥样硬化斑块的诊断仍然具有挑战性。本研究用罗丹明标记超顺磁性氧化铁（USPIO-R），并评估 USPIO-R 用于动脉粥样硬化斑块成像。载脂蛋白 E 缺陷型小鼠给予高脂饮食，并在静脉注射 USPIO-R 前后进行 MRI。随后，从小鼠中取出主动脉标本，进行荧光成像、普鲁士蓝和免疫荧光染色。注射后主动脉斑块 T2 信号丢失并持续存在，并且观察到斑块的自发荧光。斑块内 USPIO-R 的累积机制是普鲁士蓝和免疫荧光显示的巨噬细胞内化。USPIO-R 是一种很有前途的用于诊断和监测易损动脉粥样硬化斑块的双模态探针。
