Ramoji Anuradha, Ryabchykov Oleg, Galler Kerstin, Tannert Astrid, Markwart Robby, Requardt Robert P, Rubio Ignacio, Bauer Michael, Bocklitz Thomas, Popp Jürgen, Neugebauer Ute
Center for Sepsis Control and Care, Jena University Hospital, 07747 Jena, Germany.
Leibniz Institute of Photonic Technology Jena, 07745 Jena, Germany.
Immunohorizons. 2019 Feb 8;3(2):45-60. doi: 10.4049/immunohorizons.1800059.
T lymphocytes (T cells) are highly specialized members of the adaptive immune system and hold the key to the understanding the hosts' response toward invading pathogen or pathogen-associated molecular patterns such as LPS. In this study, noninvasive Raman spectroscopy is presented as a label-free method to follow LPS-induced changes in splenic T cells during acute and postacute inflammatory phases (1, 4, 10, and 30 d) with a special focus on CD4 and CD8 T cells of endotoxemic C57BL/6 mice. Raman spectral analysis reveals highest chemical differences between CD4 and CD8 T cells originating from the control and LPS-treated mice during acute inflammation, and the differences are visible up to 10 d after the LPS insult. In the postacute phase, CD4 and CD8 T cells from treated and untreated mice could not be differentiated anymore, suggesting that T cells largely regained their original status. In sum, the biological information obtained from Raman spectra agrees with immunological readouts demonstrating that Raman spectroscopy is a well-suited, label-free method for following splenic T cell activation in systemic inflammation from acute to postacute phases. The method can also be applied to directly study tissue sections as is demonstrated for spleen tissue one day after LPS insult.
T淋巴细胞（T细胞）是适应性免疫系统中高度特化的成员，是理解宿主对入侵病原体或病原体相关分子模式（如脂多糖，LPS）反应的关键。在本研究中，无创拉曼光谱被作为一种无标记方法，用于追踪内毒素血症C57BL/6小鼠在急性和急性后炎症阶段（1、4、10和30天）脾脏T细胞中LPS诱导的变化，特别关注CD4和CD8 T细胞。拉曼光谱分析显示，在急性炎症期间，来自对照小鼠和LPS处理小鼠的CD4和CD8 T细胞之间存在最大的化学差异，并且这些差异在LPS刺激后长达10天仍可见。在急性后阶段，来自处理和未处理小鼠的CD4和CD8 T细胞不再能够区分，这表明T细胞在很大程度上恢复了其原始状态。总之，从拉曼光谱获得的生物学信息与免疫学读数一致，表明拉曼光谱是一种非常适合的无标记方法，用于追踪全身炎症从急性期到急性后期脾脏T细胞的激活。该方法也可直接应用于研究组织切片，如在LPS刺激后一天对脾脏组织的研究所示。
