Salehi Hamideh, Ramoji Anuradha, Mougari Said, Merida Peggy, Neyret Aymeric, Popp Jurgen, Horvat Branka, Muriaux Delphine, Cuisinier Frederic
LBN, University of Montpellier, Montpellier, France.
Friedrich-Schiller-University Jena, Institute of Physical Chemistry and Abbe Center of Photonics (IPC), Helmholtzweg 4, D-07743 Jena, Germany.
Commun Chem. 2022;5(1):85. doi: 10.1038/s42004-022-00702-7. Epub 2022 Jul 25.
SARS-CoV-2 infection remains spread worldwide and requires a better understanding of virus-host interactions. Here, we analyzed biochemical modifications due to SARS-CoV-2 infection in cells by confocal Raman microscopy. Obtained results were compared with the infection with another RNA virus, the measles virus. Our results have demonstrated a virus-specific Raman molecular signature, reflecting intracellular modification during each infection. Advanced data analysis has been used to distinguish non-infected versus infected cells for two RNA viruses. Further, classification between non-infected and SARS-CoV-2 and measles virus-infected cells yielded an accuracy of 98.9 and 97.2 respectively, with a significant increase of the essential amino-acid tryptophan in SARS-CoV-2-infected cells. These results present proof of concept for the application of Raman spectroscopy to study virus-host interaction and to identify factors that contribute to the efficient SARS-CoV-2 infection and may thus provide novel insights on viral pathogenesis, targets of therapeutic intervention and development of new COVID-19 biomarkers.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染仍在全球范围内传播，因此需要更好地了解病毒与宿主之间的相互作用。在此，我们通过共聚焦拉曼显微镜分析了SARS-CoV-2感染细胞后引起的生化修饰。将获得的结果与另一种RNA病毒——麻疹病毒感染的情况进行了比较。我们的结果显示了一种病毒特异性的拉曼分子特征，反映了每次感染过程中的细胞内修饰。先进的数据分析已被用于区分两种RNA病毒的未感染细胞和感染细胞。此外，对未感染细胞以及感染SARS-CoV-2和麻疹病毒的细胞进行分类，准确率分别为98.9%和97.2%，且在感染SARS-CoV-2的细胞中必需氨基酸色氨酸显著增加。这些结果为拉曼光谱在研究病毒与宿主相互作用以及识别促成SARS-CoV-2有效感染的因素方面的应用提供了概念验证，从而可能为病毒发病机制、治疗干预靶点以及新型COVID-19生物标志物的开发提供新的见解。
