Ishida Y, Azuno Y, Tanaka H, Tanaka M, Miyamura S, Kaneko T, Matsumoto N
Third Department of Internal Medicine, Yamaguchi University, School of Medicine, Ube-shi, Japan.
Acta Haematol. 1988;80(1):1-7. doi: 10.1159/000205586.
Colony-stimulating factor (CSF) was partially purified from urine of patients with aplastic anemia using DEAE-cellulose and concanavalin A-Sepharose. This partially purified CSF caused significant neutrophilia in the peripheral blood of normal mice by (a) single or continual intraperitoneal injection(s) in vivo, and also revealed a specific activity of 1.4 x 10(3) U/absorbance unit (AU) at 280 nm in vitro, with less than 1 ng/AU endotoxin. In addition, this CSF induced faster recoveries of neutrophils in the peripheral blood and progenitor spleen cells of cyclophosphamide (CY)-treated mice. These findings suggest that the CSF used in this study accelerated the differentiation of the granulocytic cells and the proliferation of granulocyte colony-forming units in the spleen. These effects contributed to a rapid recovery from neutropenia in mice treated with CY.
采用二乙氨基乙基纤维素（DEAE - 纤维素）和伴刀豆球蛋白A - 琼脂糖凝胶（ConA - Sepharose）从再生障碍性贫血患者的尿液中部分纯化集落刺激因子（CSF）。这种部分纯化的CSF通过在正常小鼠体内单次或连续腹腔注射，可使外周血中出现显著的中性粒细胞增多，并且在体外280nm处还显示出1.4×10³U/吸光度单位（AU）的比活性，内毒素含量低于1ng/AU。此外，这种CSF可使环磷酰胺（CY）处理的小鼠外周血中性粒细胞和祖脾细胞更快恢复。这些发现表明，本研究中使用的CSF加速了粒细胞的分化以及脾脏中粒细胞集落形成单位的增殖。这些作用有助于CY处理的小鼠从嗜中性白血球减少症中快速恢复。
