Welte K, Bonilla M A, Gillio A P, Boone T C, Potter G K, Gabrilove J L, Moore M A, O'Reilly R J, Souza L M
J Exp Med. 1987 Apr 1;165(4):941-8. doi: 10.1084/jem.165.4.941.
We examined the in vivo effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) in primates (cynomolgus monkeys) treated with subcutaneous doses of rhG-CSF for 14-28 d. A dose-dependent increase in the peripheral white blood cells (WBC) was seen, reaching a plateau after 1 wk of rhG-CSF treatment. The elevation of WBC was due to an increase in the absolute neutrophil count. These results demonstrate that rhG-CSF is a potent granulopoietic growth and differentiation factor in vivo. In cyclophosphamide (CY)-induced myelosuppression, rhG-CSF was able to shorten the time period of WBC recovery in two treated monkeys to 1 wk, as compared to more than 4 wk for the control monkey. Its ability to significantly shorten the period of chemotherapy-induced bone marrow hypoplasia may allow clinicians to increase the frequency or dosage of chemotherapeutic agents. In addition, the increase in absolute numbers of functionally active neutrophils may have a profound effect in the rate and severity of neutropenia-related sepsis. Furthermore, the activities reported here indicate a potential role for rhG-CSF in the treatment of patients with myelodysplastic syndrome, congenital agranulocytosis, radiation-induced myelosuppression, and bone marrow transplantation.
我们研究了皮下注射重组人粒细胞集落刺激因子（rhG-CSF）14 - 28天对灵长类动物（食蟹猴）的体内作用。外周血白细胞（WBC）呈剂量依赖性增加，rhG-CSF治疗1周后达到平台期。白细胞升高是由于绝对中性粒细胞计数增加。这些结果表明，rhG-CSF在体内是一种有效的粒细胞生成生长和分化因子。在环磷酰胺（CY）诱导的骨髓抑制中，与对照猴超过4周的时间相比，rhG-CSF能够将两只治疗猴的白细胞恢复时间缩短至1周。其显著缩短化疗诱导的骨髓发育不全期的能力可能使临床医生能够增加化疗药物的频率或剂量。此外，功能活跃的中性粒细胞绝对数量的增加可能对中性粒细胞减少相关败血症的发生率和严重程度产生深远影响。此外，此处报道的活性表明rhG-CSF在治疗骨髓增生异常综合征、先天性粒细胞缺乏症、辐射诱导的骨髓抑制和骨髓移植患者中具有潜在作用。
