Gag 蛋白 p8 区的一个 PLPPV 序列为鼠乳腺瘤病毒提供晚期结构域功能。
A PLPPV sequence in the p8 region of Gag provides late domain function for mouse mammary tumor virus.
机构信息
AIDS and Cancer Virus Program, National Cancer Institute at Frederick, Frederick, MD, 21702-1201, USA.
Advanced Technology Program, National Cancer Institute at Frederick, Frederick, MD, 21702-1201, USA.
出版信息
Virology. 2019 Sep;535:272-278. doi: 10.1016/j.virol.2019.07.015. Epub 2019 Jul 19.
The late (L) domain sequence used by mouse mammary tumor virus (MMTV) remains undefined. Similar to other L domain-containing proteins, MMTV p8 and p14 proteins are monoubiquitinated, suggesting L domain function. Site-directed mutagenesis of p8, PLPPV, and p14, PLPPL, sequences in MMTV Gag revealed a requirement only for the PLPPV sequence in virion release in a position-dependent manner. Electron microscopy of a defective Gag mutant confirmed an L domain budding defect morphology. The equine infectious anemia virus (EIAV) YPDL core L domain sequence and PLPPV provided L domain function in reciprocal MMTV and EIAV Gag exchange mutants, respectively. Alanine scanning of the PLPPV sequence revealed a strict requirement for the valine residue but only minor requirements for any one of the other residues. Thus, PLPPV provides MMTV L domain function, representing a fourth type of retroviral L domain that enables MMTV Gag proteins to co-opt cellular budding pathways for release.
鼠乳腺肿瘤病毒（MMTV）使用的晚期（L）结构域序列尚未确定。与其他含有 L 结构域的蛋白质一样，MMTV p8 和 p14 蛋白被单泛素化，表明 L 结构域的功能。对 MMTV Gag 中的 p8、PLPPV 和 p14、PLPPL 序列进行定点突变，发现只有 PLPPV 序列以位置依赖的方式对病毒粒子释放有要求。对有缺陷的 Gag 突变体的电子显微镜检查证实了 L 结构域出芽缺陷的形态。马传染性贫血病毒（EIAV）的 YPDL 核心 L 结构域序列和 PLPPV 分别在相互的 MMTV 和 EIAV Gag 交换突变体中提供了 L 结构域功能。对 PLPPV 序列进行丙氨酸扫描表明，缬氨酸残基的要求非常严格，但其他任何一个残基的要求都很低。因此，PLPPV 提供了 MMTV L 结构域功能，代表了第四种能够使 MMTV Gag 蛋白共同利用细胞出芽途径进行释放的逆转录病毒 L 结构域。
Zotero 插件