State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Department of Oral Anatomy and Physiology and TMD, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Radiology, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
Oral Dis. 2019 Oct;25(7):1759-1768. doi: 10.1111/odi.13168. Epub 2019 Aug 19.
To detect whether early growth response 1 (EGR1) in peripheral blood leucocytes (PBLs) indicates temporomandibular joint (TMJ) osteoarthritis (OA) lesions.
Egr1 mRNA expression levels in PBLs were detected in eight malocclusion patients without temporomandibular disorder (TMD) signs and 16 malocclusion patients with clinical TMD signs with (eight) or without (eight) imaging signs of TMJ OA. Twelve 6-week-old rats were randomized to a control group and a unilateral anterior crossbite (UAC) group and were sampled at 4 weeks. The Egr1 mRNA expression levels in PBLs and protein expression levels in different orofacial tissues were measured.
Patients with TMD signs with/without TMJ OA diagnosis showed lower Egr1 mRNA expression levels in PBLs than patients without TMD signs. The lower Egr1 mRNA expression was also found in the PBLs of UAC rats, which were induced to exhibit early histo-morphological signs of TMJ OA lesions. In subchondral bone of UAC rats, EGR1 protein expression was decreased, co-localization of EGR1 with osterix or dentin matrix protein-1 was identified, and the number of EGR1 and osterix double-positive cells was reduced (all p < .05).
Egr1 reduction in PBLs potentially indicates subchondral bone OA lesions at an early stage.
检测外周血白细胞（PBL）中早期生长反应 1（EGR1）是否预示颞下颌关节（TMJ）骨关节炎（OA）病变。
检测 8 例无颞下颌关节紊乱病（TMD）征象的错牙合患者和 16 例有临床 TMD 征象且（8 例）或无（8 例）TMJ OA 影像学征象的错牙合患者 PBL 中 Egr1 mRNA 表达水平。将 12 只 6 周龄大鼠随机分为对照组和单侧前牙反牙合（UAC）组，4 周时取样。测量 PBL 中 Egr1 mRNA 表达水平和不同口颌面组织中蛋白表达水平。
有 TMD 征象伴/不伴 TMJ OA 诊断的患者 PBL 中 Egr1 mRNA 表达水平低于无 TMD 征象的患者。UAC 大鼠 PBL 中也发现 Egr1 mRNA 表达水平降低，这些大鼠表现出 TMJ OA 病变的早期组织形态学征象。UAC 大鼠软骨下骨中 EGR1 蛋白表达减少，与成骨特异性转录因子 2（osterix）或牙本质基质蛋白 1（dentin matrix protein-1）共定位，EGR1 和 osterix 双阳性细胞数量减少（均 p<0.05）。
PBL 中 Egr1 减少可能预示早期软骨下骨 OA 病变。
