Li Wei, Li Yang, Qian Hai-Long, Zhao Xu, Yang Cheng-Xiong, Yan Xiu-Ping
College of Chemistry, Research Center for Analytical Sciences, Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, 94 Weijin Road, Tianjin, 300071, China.
State Key Laboratory of Food Science and Technology (Jiangnan University), International Joint Laboratory on Food Safety, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Talanta. 2019 Nov 1;204:224-228. doi: 10.1016/j.talanta.2019.05.086. Epub 2019 May 24.
Gold nanoparticles (AuNPs) without surface capping agents are easily aggregated owing to their high surface energy, leading to an unexpected decrease of their catalytic activity. Herein, we report gold nanoparticles decorated covalent organic framework (COF) as mimetic peroxidase for colorimetric detection of mercury. 1,3,5-Tris-(4-formyl-phenyl)triazine (PT) and 4, 4'-azodianiline (Azo) was employed as the monomers to prepare a novel imine based COF PTAzo under solvothermal conditions. AuNPs are further decorated into the PTAzo to form COF-AuNPs hybrid via a citrate reducing method. The COF-AuNPs show high stability, and exhibits enhanced peroxidase mimetic activity in the presence of Hg. The Hg concentration dependent peroxidase mimetic activity of COF-AuNPs enables the development of a sensitive and selective method for detection of Hg in aqueous solution. The developed method gives good linearity in the range of 5-300 nM with the limit of detection of 0.75 nM. The results show that Hg-enhanced peroxidase mimetic activity of COF-AuNPs offers great potential for the detection of Hg in real samples.
没有表面封端剂的金纳米颗粒（AuNPs）由于其高表面能而容易聚集，导致其催化活性意外降低。在此，我们报道了用共价有机框架（COF）修饰的金纳米颗粒作为用于比色检测汞的模拟过氧化物酶。采用1,3,5-三（4-甲酰基苯基）三嗪（PT）和4,4'-偶氮二苯胺（Azo）作为单体，在溶剂热条件下制备了一种新型的基于亚胺的COF PTAzo。通过柠檬酸盐还原法将AuNPs进一步修饰到PTAzo中，形成COF-AuNPs杂化物。COF-AuNPs表现出高稳定性，并且在汞存在下表现出增强的过氧化物酶模拟活性。COF-AuNPs的汞浓度依赖性过氧化物酶模拟活性使得能够开发一种灵敏且选择性的水溶液中汞检测方法。所开发的方法在5-300 nM范围内具有良好的线性，检测限为0.75 nM。结果表明，COF-AuNPs的汞增强过氧化物酶模拟活性为实际样品中汞的检测提供了巨大潜力。
