Landi Simone, Capasso Giorgia, Ben Azaiez Fatma Ezzahra, Jallouli Salma, Ayadi Sawsen, Trifa Youssef, Esposito Sergio
Dipartimento di Biologia, Università di Napoli ''Federico II'', Via Cinthia, I-80126 Napoli, Italy.
Department of Agronomy and Plant Biotechnology, National Institute of Agronomy, 1082 Tunis, Tunisia.
Plants (Basel). 2019 Jul 26;8(8):248. doi: 10.3390/plants8080248.
In this work, the involvement of heat shock proteins (HSP70) in barley () has been studied in response to drought and salinity. Thus, 3 barley genotypes usually cultivated and/or selected in Italy, 3 Middle East/North Africa landraces and genotypes and 1 improved genotype from ICARDA have been studied to identify those varieties showing the best stress response. Preliminarily, a bioinformatic characterization of the HSP70s protein family in barley has been made by using annotated Arabidopsis protein sequences. This study identified 20 putative HSP70s orthologs in the barley genome. The construction of un-rooted phylogenetic trees showed the partition into four main branches, and multiple subcellular localizations. The enhanced HSP70s presence upon salt and drought stress was investigated by both immunoblotting and expression analyses. It is worth noting the Northern Africa landraces showed peculiar tolerance behavior versus drought and salt stresses. The drought and salinity conditions indicated the involvement of specific HSP70s to counteract abiotic stress. Particularly, the expression of cytosolic MLOC_67581, mitochondrial MLOC_50972, and encoding for HSP70 isoforms showed different expressions and occurrence upon stress. Therefore, genotypes originated in the semi-arid area of the Mediterranean area can represent an important genetic source for the improvement of commonly cultivated high-yielding varieties.
在这项研究中，对大麦中热休克蛋白（HSP70）在干旱和盐胁迫响应中的作用进行了研究。为此，对通常在意大利种植和/或选育的3个大麦基因型、3个中东/北非地方品种和基因型以及国际干旱地区农业研究中心（ICARDA）的1个改良基因型进行了研究，以确定那些表现出最佳胁迫响应的品种。首先，利用注释的拟南芥蛋白质序列对大麦中HSP70s蛋白家族进行了生物信息学表征。本研究在大麦基因组中鉴定出20个推定的HSP70直系同源物。无根系统发育树的构建显示其分为四个主要分支以及多种亚细胞定位。通过免疫印迹和表达分析研究了盐胁迫和干旱胁迫下HSP70s的表达增强情况。值得注意的是，北非地方品种对干旱和盐胁迫表现出特殊的耐受行为。干旱和盐胁迫条件表明特定HSP70s参与了对非生物胁迫的抵抗。特别是，胞质MLOC_67581、线粒体MLOC_50972以及编码HSP70亚型的基因在胁迫下表现出不同的表达和出现情况。因此，起源于地中海地区半干旱地区的基因型可成为改良常见种植的高产品种的重要遗传资源。
