Chappell C L, Dresden M H
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
Am J Trop Med Hyg. 1988 Jul;39(1):66-73. doi: 10.4269/ajtmh.1988.39.66.
Indirect immunofluorescence of Schistosoma mansoni adult worm sections has revealed that the early immunoglobulin response is directed toward the parasite digestive tract. One of the components of the worm gut is a cysteine proteinase which degrades host hemoglobin ingested by the parasite. In this report the purified proteinase (SMw32) was used in ELISA and immunoblot analyses to study the specific antibody response during the course of an acute infection. We have found high titer IgG antibody in S. mansoni infected, but not uninfected, mice. The anti-proteinase response involves IgM, IgG1, IgG2a, and IgE isotypes. Total IgM and IgG levels increased by week 3 post-infection and remained elevated throughout the study (7 weeks). Increased titers (IgM, IgG) of specific anti-proteinase were also apparent by week 3 post-infection, long before fecal eggs were detectable. Mean anti-proteinase IgG stabilized at high titer by week 5 post-infection, while IgM titers decreased to near background levels. Anti-proteinase IgE was first detectable at week 4 and reached peak titers by weeks 6 and 7. The strong antibody response to the purified SMw32 proteinase is consistent with the early reactivity of S. mansoni infected mice and humans to a 31 kDa component of the worm gut described by others.
曼氏血吸虫成虫切片的间接免疫荧光显示，早期免疫球蛋白反应针对寄生虫的消化道。虫体肠道的成分之一是一种半胱氨酸蛋白酶，它能降解寄生虫摄取的宿主血红蛋白。在本报告中，纯化的蛋白酶（SMw32）用于酶联免疫吸附测定（ELISA）和免疫印迹分析，以研究急性感染过程中的特异性抗体反应。我们在感染曼氏血吸虫但未感染的小鼠中发现了高滴度的IgG抗体。抗蛋白酶反应涉及IgM、IgG1、IgG2a和IgE同种型。总IgM和IgG水平在感染后第3周升高，并在整个研究期间（7周）保持升高。感染后第3周，特异性抗蛋白酶的滴度（IgM、IgG）也明显升高，远早于粪便中可检测到虫卵。感染后第5周，平均抗蛋白酶IgG稳定在高滴度，而IgM滴度降至接近背景水平。抗蛋白酶IgE在第4周首次可检测到，并在第6周和第7周达到峰值滴度。对纯化的SMw32蛋白酶的强烈抗体反应与曼氏血吸虫感染的小鼠和人类对其他人描述为虫体肠道31 kDa成分的早期反应性一致。
