Cowen B S, Braune M O
Department of Veterinary Science, College of Agriculture, Pennsylvania State University, University Park 16802.
Avian Dis. 1988 Apr-Jun;32(2):282-97.
Seven of nine avian virus families tested (Birnaviridae, Coronaviridae, Herpesviridae, Paramyxoviridae, Poxviridae, Reoviridae, and Retroviridae) were found to replicate in a quail fibroblast cell line, designated QT35, resulting in a cytopathic effect (CPE) visible with the naked eye or by low-power microscopy. In comparison, only one (Paramyxoviridae) of seven mammalian virus families tested produced an observable CPE. Cytopathic changes induced by examined viruses were round cell, syncytial, and focus formation. Trypsin did not promote cytopathic changes by selected CPE-negative avian and mammalian viruses in QT35 cells. Several avian viruses (infectious bursal disease virus, Newcastle disease virus, Canary pox virus, and reovirus) formed plaques under agar. Avian reovirus and infectious bursal disease virus produced similar titers in chicken embryo fibroblast (CEF) and QT35 cell cultures. Chicken-egg-yolk neutralizing-antibody titers to IBDV were comparable in CEF and QT35 cell-culture systems.
在接受检测的9个禽病毒科中（双RNA病毒科、冠状病毒科、疱疹病毒科、副粘病毒科、痘病毒科、呼肠孤病毒科和逆转录病毒科），有7个科的病毒被发现可在一种名为QT35的鹌鹑成纤维细胞系中复制，从而产生肉眼可见或通过低倍显微镜可观察到的细胞病变效应（CPE）。相比之下，在接受检测的7个哺乳动物病毒科中，只有1个科（副粘病毒科）产生了可观察到的CPE。所检测病毒诱导的细胞病变变化包括圆形细胞、多核巨细胞和病灶形成。胰蛋白酶不会促进选定的CPE阴性禽病毒和哺乳动物病毒在QT35细胞中产生细胞病变变化。几种禽病毒（传染性法氏囊病病毒、新城疫病毒、金丝雀痘病毒和呼肠孤病毒）在琼脂下形成了蚀斑。禽呼肠孤病毒和传染性法氏囊病病毒在鸡胚成纤维细胞（CEF）和QT35细胞培养物中的滴度相似。在CEF和QT35细胞培养系统中，针对传染性法氏囊病病毒的鸡卵黄中和抗体滴度相当。
