Cho B R
Avian Dis. 1984 Jan-Mar;28(1):261-5.
Reticuloendotheliosis virus (REV) strains T and CS consistently induced focus formation in a quail fibroblast cell line designated QT35 that was maintained under agarose overlay following inoculation of REV. The foci were more distinct and better circumscribed than those that developed in QT35 cultures under fluid medium. The focus formation in QT35 cultures under agarose overlay was inhibited by REV antiserum, and there were a highly significant correlation (r = 0.966, P less than 0.005) and a linear relationship between the number of foci that developed and relative virus concentration inoculated. This provides a valid focus assay for REVs.
网状内皮组织增生症病毒（REV）毒株T和CS在一种名为QT35的鹌鹑成纤维细胞系中持续诱导形成病灶。接种REV后，该细胞系在琼脂糖覆盖下进行培养。与在液体培养基中培养的QT35细胞所形成的病灶相比，这些病灶更加明显且边界更清晰。琼脂糖覆盖下的QT35细胞培养物中的病灶形成受到REV抗血清的抑制，并且所形成的病灶数量与接种的相对病毒浓度之间存在高度显著的相关性（r = 0.966，P < 0.005）和线性关系。这为REV提供了一种有效的病灶检测方法。
