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磷脂酰肌醇特异性磷脂酶D：一种生成第二信使的途径。

Phosphatidylinositol-specific phospholipase D: a pathway for generation of a second messenger.

作者信息

Balsinde J, Diez E, Mollinedo F

机构信息

Unidad de Biomembranas Centro de Investigaciones Biológicas, C.S.I.C., Madrid, Spain.

出版信息

Biochem Biophys Res Commun. 1988 Jul 29;154(2):502-8. doi: 10.1016/0006-291x(88)90168-4.

DOI:10.1016/0006-291x(88)90168-4
PMID:3135804
Abstract

We have found a phospholipase D activity in the postnuclear fraction of human neutrophils which is stimulated by incubation of cells with the calcium ionophore A23187. The phospholipase D activity was assessed by both phosphatidate formation and free inositol release from phosphatidylinositol substrate. The phospholipase D activity shows an optimum pH of 7.5 and hydrolyzes specifically phosphatidylinositol. These results suggest that this phosphatidylinositol-specific phospholipase D can play a role in cell activating process.

摘要

我们在人中性粒细胞的核后组分中发现了一种磷脂酶D活性，用钙离子载体A23187孵育细胞可刺激该活性。通过磷脂酸形成和从磷脂酰肌醇底物释放游离肌醇来评估磷脂酶D活性。该磷脂酶D活性的最适pH为7.5，且特异性水解磷脂酰肌醇。这些结果表明，这种磷脂酰肌醇特异性磷脂酶D可能在细胞激活过程中发挥作用。

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Phosphatidylinositol-specific phospholipase D: a pathway for generation of a second messenger.磷脂酰肌醇特异性磷脂酶D：一种生成第二信使的途径。
Biochem Biophys Res Commun. 1988 Jul 29;154(2):502-8. doi: 10.1016/0006-291x(88)90168-4.
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Biochemical characterization of phospholipase D activity from human neutrophils.
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v-Src activates a unique phospholipase D activity that can be distinguished from the phospholipase D activity activated by phorbol esters.
v-Src激活一种独特的磷脂酶D活性，该活性可与佛波酯激活的磷脂酶D活性区分开来。
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The substrate specificity of brain microsomal phospholipase D.脑微粒体磷脂酶D的底物特异性
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Accumulation of inositol polyphosphate isomers in agonist-stimulated cerebral-cortex slices. Comparison with metabolic profiles in cell-free preparations.激动剂刺激的大脑皮质切片中肌醇多磷酸异构体的积累。与无细胞制剂中代谢谱的比较。
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