Geller-Bernstein C, Mazurek N, Berger G, Hemerich S, Loyter A, Berebi A, Ortega E, Corcia A, Rivnay B, Pecht I
Weizmann Institut Rehovot, Israel.
Allerg Immunol (Paris). 1987 Feb;19(2):56-8.
Previous research has shown that cromolyn (disodium cromoglycate) binds specifically to the membrane of rat mast and basophil leukemia (RBL) cells, forming a ternary complex with Ca++, blocking of the Ca++ results in inhibition of histamine release upon immunologic triggering. The specific cromolyn binding protein (CBP) has been isolated by using its high affinity form cromolyn. Specific monoclonal anti CBP antibodies have been obtained in mice and polyclonal anti-cromolyn antibodies in rabbits. These antibodies have been used for further purification and characterization of the CBP. Experiments on RBL cell lines have shown that CBP is essential for Ca++ influx and histamine liberation upon immunologic triggering by these cells. Variant CBP deficient RBL do not take up Ca++ and do not degranulate in response to immunologic triggering but their ability to respond normally can be induced by incorporating CBP into their membranes with help of Sendai virus carrier vesicles. This shows that CBP plays a crucial role for the RBL cells Ca++ influx and histamine release following IgE crosslinking.
先前的研究表明，色甘酸钠（色甘酸二钠）能特异性地结合大鼠肥大细胞和嗜碱性粒细胞白血病（RBL）细胞的膜，与钙离子形成三元复合物，阻断钙离子会抑制免疫触发时组胺的释放。通过使用其高亲和力形式的色甘酸钠，已分离出特异性色甘酸钠结合蛋白（CBP）。在小鼠中获得了特异性抗CBP单克隆抗体，在兔中获得了多克隆抗色甘酸钠抗体。这些抗体已用于CBP的进一步纯化和表征。对RBL细胞系的实验表明，CBP对于这些细胞免疫触发时的钙离子内流和组胺释放至关重要。缺乏CBP变体的RBL细胞不摄取钙离子，对免疫触发无脱颗粒反应，但通过仙台病毒载体囊泡将CBP掺入其膜中，可诱导它们正常反应的能力。这表明CBP在IgE交联后RBL细胞的钙离子内流和组胺释放中起关键作用。
