Thomas G, Thomas L, Thorne B A, Herbert E
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201.
Biochimie. 1988 Jan;70(1):89-97. doi: 10.1016/0300-9084(88)90163-0.
The cell-type specific processing of human pro-enkephalin was determined using a vaccinia recombinant (VV:hPE). The results show that all cell types infected with VV:hPE efficiently synthesize pro-enkephalin following cleavage of the signal peptide after Ala24. In addition, pro-enkephalin is shown to undergo N-linked glycosylation as well as other post-translational modifications. However, only one cell line. AtT-20, was able to efficiently cleave pro-enkephalin to smaller peptides including Met-enkephalin. Some results have been previously reported (Science 232, 1641-1643).
