Departamento Micología, INEI ANLIS "Dr. Carlos G. Malbrán", Av. Vélez Sarsfield 563, CP 1281, Buenos Aires, Argentina.
Cátedra Micología Médica e Industrial, Universidad Nacional de La Plata, Calle 60 y 119. La Plata, CP 1900, Buenos Aires, Argentina.
Mycopathologia. 2019 Oct;184(5):615-623. doi: 10.1007/s11046-019-00364-5. Epub 2019 Jul 29.
We evaluated the in vitro antifungal activity of essential oils obtained from the aromatic plants Laurus nobilis, Thymus vulgaris, Mentha piperita, Cymbopogon citratus and Lippia junelliana against the following Candida species isolated from clinical samples: C. krusei (n = 10); C. albicans (n = 50); C. glabrata (n = 70) and C. parapsilosis (n = 80). The minimal inhibitory concentration (MIC) was determined according to EDef 7.3.1 document from EUCAST. Amphotericin B and fluconazole were the antifungal drugs used as inhibition control. The concentration ranges evaluated were 0.4-800 and 0.03-128 mg l for essential oils and antifungal drugs, respectively. MIC and MIC, mode and ranges were calculated. All the Candida spp. evaluated were susceptible to amphotericin B (MIC ≤ 1 mg l), while fluconazole was inactive for C. krusei (MIC ≥ 32 mg l) and intermediate for C. glabrata (MIC≤ 32 mg l). The essential oils showed antifungal activity on Candida spp. tested with MIC values ranging from 0.8 to 800 mg l. In general, the most active essential oils were L. nobilis and T. vulgaris (MIC 0.8-0.16 mg l), and the least active was C. officinalis (MIC 400-800 mg l). C. krusei was inhibited by 5/6 of the essential oils evaluated, and C. glabrata was the least susceptible one. This in vitro study confirms the antifungal activity of these six essential oils assayed which could be a potential source of new molecules useful to control fungal infections caused by some Candida species, including those resistant to antifungal drugs.
我们评估了从芳香植物月桂、百里香、薄荷、柠檬香茅和 Lippia junelliana 中获得的精油对以下从临床样本中分离出的念珠菌属物种的体外抗真菌活性：C. krusei（n=10）；C. albicans（n=50）；C. glabrata（n=70）和 C. parapsilosis（n=80）。根据 EUCAST 的 EDef 7.3.1 文件确定最小抑菌浓度（MIC）。两性霉素 B 和氟康唑是用作抑制对照的抗真菌药物。评估的浓度范围分别为 0.4-800 和 0.03-128 mg l 用于精油和抗真菌药物。计算 MIC 和 MIC、模式和范围。评估的所有念珠菌属物种均对两性霉素 B 敏感（MIC≤1 mg l），而氟康唑对 C. krusei 无效（MIC≥32 mg l），对 C. glabrata 则为中介（MIC≤32 mg l）。精油对测试的念珠菌属物种表现出抗真菌活性，MIC 值范围为 0.8 至 800 mg l。一般来说，最活跃的精油是月桂和百里香（MIC 0.8-0.16 mg l），而最不活跃的是 C. officinalis（MIC 400-800 mg l）。C. krusei 被评估的 5/6 种精油抑制，而 C. glabrata 是最不敏感的一种。这项体外研究证实了所测试的六种精油的抗真菌活性，它们可能是控制某些念珠菌属物种引起的真菌感染的新分子的潜在来源，包括对抗真菌药物耐药的那些。
