Shukla Y, Antony M, Kumar S, Mehrotra N K
Laboratory of Environmental Carcinogenesis, Industrial Toxicology Research Centre, Lucknow, India.
Carcinogenesis. 1988 Aug;9(8):1511-2. doi: 10.1093/carcin/9.8.1511.
In this study the tumour-promoting activity of a carbamate fungicide, mancozeb, has been observed following topical application on mouse skin in a two-stage initiation--promotion protocol for carcinogenesis. Female Swiss albino mice were initiated with a single subcarcinogenic dose (52 micrograms) of 7,12-dimethylbenz[a]anthracene painted on the interscapular region. Seven days after initiation the mice underwent topical application of mancozeb (100 mg/kg body wt) three times per week as promoter. Development of tumours was observed after 12 weeks of mancozeb application in 1/14 animals and 100% tumorigenesis was recorded after 17 weeks of mancozeb application. On histological examination, these tumours were found mostly to be benign in nature, e.g. squamous cell papillomas and keratocanthomas.
在本研究中，按照两阶段致癌启动 - 促进方案，在小鼠皮肤上局部应用氨基甲酸酯类杀菌剂代森锰锌后，观察到了其促肿瘤活性。雌性瑞士白化小鼠在肩胛间区域涂抹单次亚致癌剂量（52微克）的7,12 - 二甲基苯并[a]蒽进行启动。启动7天后，小鼠每周3次局部应用代森锰锌（100毫克/千克体重）作为促进剂。应用代森锰锌12周后，在1/14的动物中观察到肿瘤发生，应用代森锰锌17周后记录到100%的肿瘤发生率。组织学检查发现，这些肿瘤大多为良性，如鳞状细胞乳头状瘤和角化棘皮瘤。
