Tai Yan-Ni, Weng Yan-Hong, Zhang Su-Ping, Xu Wen, Li Xiao-Yan, Lin Qing-Qing, Chu Ke-Dan, Wu Shui-Sheng
School of Pharmacy, Fujian University of Traditional Chinese Medicine Fuzhou 350122, China Centre of Biomedical Research & Development, Fujian University of Traditional Chinese Medicine Fuzhou 350122, China.
School of Pharmacy, Fujian University of Traditional Chinese Medicine Fuzhou 350122, China.
Zhongguo Zhong Yao Za Zhi. 2019 Jun;44(11):2292-2307. doi: 10.19540/j.cnki.cjcmm.20190321.205.
The present study is to establish a quantitative analysis of multi-components by single marker(QAMS) for determining contents of seven compositions in Alismatis Rhizoma, alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B, alisol B 23-acetate and 11-deoxy-alisol B. Six relative correction factors(RCFs) of alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B and 11-deoxy-alisol B were established in the UPLC method with alisol B 23-acetate as the internal standard, which was to calculate the mass fraction of each. The mass fraction of seven effective constituents in Alismatis Rhizoma was calculated by the external standard method(ESM) at the same time. Compared with the content results determined by the ESM and QAMS, the feasibility and accuracy of QAMS method were verified. Within the linear range, the RCFs of alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B, 11-deoxy-alisol B were 0.946, 4.183, 0.915, 1.039, 0.923 and 1.244, respectively, with good repeatability in different experimental conditions. There was no significant difference between the QAMS method and ESM method. Then, QAMS method was applied to determination of the different degree Alismatis Rhizoma from different areas. As a result, the concentrations of 7 components have differences in different areas, but no significant differences in different grades. The QAMS method is feasible and accurate for the determination of the seven chemical compositions, and which can be used for quality control of Alismatis Rhizoma.
本研究旨在建立一种单指标多成分定量分析（QAMS）方法，用于测定泽泻中7种成分，即泽泻醇氧化物、泽泻醇C 23 - 乙酸酯、泽泻醇A、泽泻醇、泽泻醇B、泽泻醇B 23 - 乙酸酯和11 - 去氧泽泻醇B的含量。以泽泻醇B 23 - 乙酸酯为内标，采用超高效液相色谱法（UPLC）建立了泽泻醇氧化物、泽泻醇C 23 - 乙酸酯、泽泻醇A、泽泻醇、泽泻醇B和11 - 去氧泽泻醇B的6个相对校正因子（RCF），并计算各成分的质量分数。同时采用外标法（ESM）计算泽泻中7种有效成分的质量分数。通过比较ESM法和QAMS法测定的含量结果，验证了QAMS法的可行性和准确性。在该线性范围内，泽泻醇氧化物、泽泻醇C 23 - 乙酸酯、泽泻醇A、泽泻醇、泽泻醇B、11 - 去氧泽泻醇B的RCF分别为0.946、4.183、0.915、1.039、0.923和1.244，在不同实验条件下具有良好的重复性。QAMS法与ESM法测定结果无显著差异。随后，将QAMS法应用于不同产地、不同等级泽泻的测定。结果表明，不同产地7种成分的含量存在差异，但不同等级间无显著差异。QAMS法测定这7种化学成分可行、准确，可用于泽泻的质量控制。
