Nardin E H, Barr P J, Heimer E, Etlinger H M
Department of Medical and Molecular Parasitology, New York University School of Medicine, New York.
Eur J Immunol. 1988 Jul;18(7):1119-22. doi: 10.1002/eji.1830180722.
The antibody response of inbred and congenic strains of mice immunized with a recombinant Plasmodium vivax circumsporozoite protein was investigated. Only mice of the H-2k and H-2a haplotypes developed antibodies that react with P. vivax sporozoite in an indirect immunofluorescence assay and with the recombinant protein in enzyme-linked immunosorbent assay. Congenic recombinant strains were used to map Ir gene control to the I-Ak locus. A similar pattern of genetic restriction was found when congenic strains were immunized with a carrier-free synthetic peptide corresponding to the repeat region of the P. vivax circumsporozoite protein. The results suggest that the ability of T helper cells to recognize a T cell epitope(s) in the repeat region of the P. vivax circumsporozoite protein is genetically restricted.
研究了用重组间日疟原虫环子孢子蛋白免疫的近交系和同类系小鼠的抗体反应。只有H-2k和H-2a单倍型的小鼠产生了在间接免疫荧光试验中与间日疟原虫子孢子反应以及在酶联免疫吸附试验中与重组蛋白反应的抗体。利用同类重组系将Ir基因控制定位到I-Ak位点。当用与间日疟原虫环子孢子蛋白重复区域相对应的无载体合成肽免疫同类系时，发现了类似的遗传限制模式。结果表明，T辅助细胞识别间日疟原虫环子孢子蛋白重复区域中T细胞表位的能力受到遗传限制。
