Khalili-Tanha Ghazaleh, Barzegar Ali, Nikbakhsh Novin, Ansari-Pirsaraei Zarbakht
Department of Basic Sciences, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran.
Department of Surgery, Babol University of Medical Sciences, Babol, Iran.
Int J Prev Med. 2019 Jun 7;10:92. doi: 10.4103/ijpvm.IJPVM_57_18. eCollection 2019.
Breast cancer is one of the most frequent women malignancies in the world. The cytochrome P450 1A1 () is a key enzyme in xenobiotics metabolism. Moreover, CYP1A1 plays a critical role in the etiology of breast cancer by involving in 2-hydroxylation of estrogen. Therefore, single-nucleotide polymorphisms (SNPs) of its coding gene have been verified to be important in cancer susceptibility. The aim of the study was to evaluate the association of M2 (A2455G) includes rs1048943 of this SNP polymorphism with the risk of breast cancer in Mazandaran province.
Ninety-six breast cancer patients with known clinicopathological characters and 110 healthy women as control were genotyped for M2 polymorphisms by the restriction fragment length polymorphism technique.
The analysis of gene (polymorphism M2) showed that the frequency of homozygous wild genotypes (AA), heterozygous (AG), and mutant genotype (GG) in the patient group, respectively, 78%, 22%, and 0%, and also the frequency of genotypes AA, AG, and GG in healthy included 82%, 16%, and 2%, respectively. Statistical analysis by Logistic regression model at < 0.05 showed no significant correlation between polymorphisms in 1A1M2 and breast cancer risk (odds ratio = 0.84, confidence interval = 0.33-2.17).
The results indicated that the M2 allelic genotypes were significantly associated neither with breast cancer risk nor with clinicopathological characteristics in Mazandaran province.
乳腺癌是全球女性中最常见的恶性肿瘤之一。细胞色素P450 1A1（CYP1A1）是外源性物质代谢中的关键酶。此外，CYP1A1通过参与雌激素的2-羟化作用在乳腺癌的病因学中发挥关键作用。因此，其编码基因的单核苷酸多态性（SNP）已被证实对癌症易感性很重要。本研究的目的是评估CYP1A1 M2（A2455G）包括该SNP多态性的rs1048943与马赞德兰省乳腺癌风险的关联。
采用限制性片段长度多态性技术对96例具有已知临床病理特征的乳腺癌患者和110名健康女性作为对照进行CYP1A1 M2多态性基因分型。
对CYP1A1基因（多态性M2）的分析显示，患者组中纯合野生基因型（AA）、杂合子（AG）和突变基因型（GG）的频率分别为78%、22%和0%，健康组中基因型AA、AG和GG的频率分别为82%、16%和2%。Logistic回归模型在P<0.05时的统计分析显示，CYP1A1 M2多态性与乳腺癌风险之间无显著相关性（优势比=0.84，置信区间=0.33-2.17）。
结果表明，在马赞德兰省，CYP1A1 M2等位基因型与乳腺癌风险及临床病理特征均无显著关联。
