由pEX3及其截短的pEX3衍生物表达的融合蛋白的纯化。

Purification of fusion proteins expressed by pEX3 and a truncated pEX3 derivative.

作者信息

Kocken C H, Scheer J M, Welling G W, Welling-Wester S

机构信息

Rijksuniversiteit Groningen, Laboratorium voor Medische Microbiologie, The Netherlands.

出版信息

FEBS Lett. 1988 Aug 15;236(1):132-4. doi: 10.1016/0014-5793(88)80300-4.

DOI:10.1016/0014-5793(88)80300-4

PMID:3136038

Abstract

A derivative of the pEX3 expression vector was constructed that codes for the first 407 amino acids of the 1051 amino acids of the pEX3 fusion protein. The amount of truncated fusion protein (40 mg/g cells), obtained by expression in E. coli, was similar to that produced by the original pEX3 vector. The truncated fusion protein was purified more easily from E. coli contaminants than the original fusion protein by washing with 2 M urea and 0.5% Triton X-100.

摘要

构建了pEX3表达载体的一种衍生物，它编码pEX3融合蛋白1051个氨基酸中的前407个氨基酸。通过在大肠杆菌中表达获得的截短融合蛋白的量（40 mg/g细胞）与原始pEX3载体产生的量相似。与原始融合蛋白相比，截短的融合蛋白通过用2 M尿素和0.5% Triton X-100洗涤，更容易从大肠杆菌污染物中纯化出来。