Bingham A H
Division of Biologics, PHLS Centre for Applied Microbiology and Research, Salisbury, Wiltshire, U.K.
FEMS Microbiol Lett. 1991 Apr 15;63(2-3):239-46. doi: 10.1016/0378-1097(91)90092-o.
A series of six expression vectors, pXM184Lac.A, B, C, pXM184Z.A, B, C, based on the low copy plasmid pACYC184 that allow for expression of proteins fused to beta-galactosidase in Escherichia coli is described. A level of 50,000 units of beta-galactosidase is routinely observed and is easily identifiable on protein gels. This paper also reports the tight regulation of expression of the Trc promoter in these vectors using the LacIq repressor.
描述了一系列六个表达载体，即基于低拷贝质粒pACYC184的pXM184Lac.A、B、C、pXM184Z.A、B、C，它们可在大肠杆菌中实现与β-半乳糖苷酶融合的蛋白质的表达。通常可观察到50,000单位的β-半乳糖苷酶水平，并且在蛋白质凝胶上易于识别。本文还报道了使用LacIq阻遏物对这些载体中Trc启动子表达的严格调控。
