Bahçeci Selen, Şimşek Fatma, Eren Erdem, Aladaǧ Ibrahim
Department of Histology and Embryology, Ataturk Training and Research Hospital, Faculty of Medicine, Izmir Katip Celebi University, Izmir, Turkey.
Department of Otolaryngology-Head and Neck Surgery, Ataturk Training and Research Hospital, Faculty of Medicine, Izmir Katip Celebi University, Izmir, Turkey.
Indian J Pathol Microbiol. 2019 Jul-Sep;62(3):375-378. doi: 10.4103/IJPM.IJPM_168_18.
Nasal polyps (NP) is a common chronic inflammatory disease of the mucous membranes in the nose and paranasal sinuses. The underlying mechanisms of pathologic conditions to NP formation remains unclear. The aim of this study is to evaluate the expression of Akt and estrogen receptor (ER) in nasal polyps.
We respectively obtained 20 nasal polyp tissue and 15 concha from patients undergoing endoscopic polyp biopsy and turbinate resection. All samples were fixed in 10% formalin for 24 h and embedding in paraffin was using routine protocol for histological prepation. Sections 5 μm thick were cut and stained H&E. Tissue samples were stained with anti-ER and anti-Akt primary antibody, ER and Akt were evaluated immunohistochemically. There is a relationship between the estrogen receptor and PI3K/Akt signaling pathway in malignansy. In this study, it showed the effect of estrogen on the activation of Akt signaling pathway in nasal polyps. Mann- Whitney-U test was applied to evaluate the statistical differences between nasal polyp and control group, (P < 0.05) was accepted as significant.
In H&E stained sections we observed a lot of inflamatory cells and eosinophils in the mucosa, submucosal connective tissue and around the glandular epithelium in nasal polyps. The mucosa and submucosal connective tissue was seen normally in control group in H&E stained. We determined that ER and Akt were intensely expressed in nasal polyps. Expression is localized especially in epithelial and glandular epithelium cells and submucosal connective tissue. In contrast, expression of ER and Akt were mildly expressed in turbinate resection samples.
The expression of ER and Akt might be important factors in nasal polyp pathogenesis and may shed new light on clinical approaches in nasal polyp treatment.
鼻息肉是鼻腔和鼻窦黏膜常见的慢性炎症性疾病。鼻息肉形成的病理状况的潜在机制仍不清楚。本研究的目的是评估Akt和雌激素受体（ER）在鼻息肉中的表达。
我们分别从接受内镜息肉活检和鼻甲切除术的患者中获取了20份鼻息肉组织和15份鼻甲组织。所有样本均用10%福尔马林固定24小时，并按照常规组织学制备方案进行石蜡包埋。切成5μm厚的切片并进行苏木精-伊红（H&E）染色。组织样本用抗ER和抗Akt一抗染色，通过免疫组织化学评估ER和Akt。在恶性肿瘤中雌激素受体与PI3K/Akt信号通路之间存在关联。在本研究中，显示了雌激素对鼻息肉中Akt信号通路激活的影响。应用曼-惠特尼-U检验评估鼻息肉组和对照组之间的统计学差异，P<0.05被认为具有显著性。
在H&E染色切片中，我们观察到鼻息肉的黏膜、黏膜下结缔组织以及腺上皮周围有大量炎性细胞和嗜酸性粒细胞。在H&E染色的对照组中，黏膜和黏膜下结缔组织正常。我们确定ER和Akt在鼻息肉中强烈表达。表达尤其定位于上皮细胞、腺上皮细胞和黏膜下结缔组织。相比之下，ER和Akt在鼻甲切除样本中轻度表达。
ER和Akt的表达可能是鼻息肉发病机制中的重要因素，可能为鼻息肉治疗的临床方法提供新的思路。
