UMR 1318, Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, France.
Weed Research Laboratory, Nanjing Agricultural University, China.
PLoS One. 2019 Jul 30;14(7):e0219923. doi: 10.1371/journal.pone.0219923. eCollection 2019.
Plant cell walls development is an integrated process during which several components are deposited successively. In the cell walls in grass, the accessibility of structural polysaccharides is limited by the cell walls structure and composition mainly as a result of phenolic compounds. Here, we studied the patterns of cell walls establishment in the internode supporting the ear in three distinct maize genotypes. The developmental patterns observed in the internode cell walls in terms of its composition are reported with an emphasis on lignification, p-coumaroylation and feruloylation. We combined biochemical and histological approaches and revealed that internode cell walls development in maize before flowering is characterized by the rapid deposition of secondary cell walls components and robust lignification in both the pith and the rind. After flowering and until silage maturity, the slow deposition of secondary walls components occurs in the cortical region, and the deposited lignins are rich in β-O-4 bonds and are highly p-coumaroylated. We conclude the paper by proposing a revised spatiotemporal model based on that proposed by Terashima et al. (1993) for cell walls development in grass.
植物细胞壁的发育是一个整合的过程，在此过程中会相继沉积几种成分。在草类的细胞壁中，由于酚类化合物的存在，结构多糖的可及性受到细胞壁结构和组成的限制。在这里，我们研究了三个不同玉米基因型中支撑穗轴的节间细胞壁建立的模式。观察到的节间细胞壁在组成上的发育模式被报道，重点是木质化、对香豆酰化和阿魏酰化。我们结合了生化和组织学方法，揭示了玉米在开花前的节间细胞壁发育的特点是快速沉积次生细胞壁成分和在髓质和韧皮部都有强烈的木质化。在开花后到青贮成熟期间，次生壁成分在皮层区域缓慢沉积，沉积的木质素富含β-O-4 键，并高度对香豆酰化。我们通过提出一个基于 Terashima 等人（1993 年）提出的草类细胞壁发育的时空模型的修订版，结束了本文。
