UMR 1318, Institut Jean-Pierre Bourgin, INRA-AgroParisTech, CNRS, Universite Paris-Saclay , 78026 Versailles Cedex , France.
École Doctorale 567 Sciences du Vegetal , University Paris-Sud, University of Paris-Saclay , bat 360 , Orsay Cedex 91405 , France.
J Agric Food Chem. 2018 May 16;66(19):4800-4808. doi: 10.1021/acs.jafc.7b05755. Epub 2018 May 7.
Water supply and valorization are two urgent issues in the utilization of maize biomass in the context of climate change and replacement of fossil resources. Maximizing maize biomass valorization is of interest to make biofuel conversion competitive, and to increase forage energetic value for animal fodder. One way to estimate biomass valorization is to quantify cell wall degradability. In this study, we evaluated the impact of water supply on cell wall degradability, cell wall contents and structure, and distribution of lignified cell types in maize internodes using dedicated high-throughput tools to effectively phenotype maize internodes from 11 inbred lines under two contrasting irrigation scenarios in field trials over three years. Overall, our results clearly showed that water deficit induced significant changes in lignin content and distribution along with a reduction in lignin p-coumaroylation, thereby impacting cell wall degradability. Additionally, we also observed that responses to a water deficit varied between the lines examined, underscoring biochemical and histological target traits for plant breeding.
供水和增值是在气候变化和替代化石资源的背景下利用玉米生物量的两个紧迫问题。最大限度地提高玉米生物量的增值对于使生物燃料转化具有竞争力以及提高饲料的能量价值具有重要意义。估计生物质增值的一种方法是量化细胞壁可降解性。在这项研究中，我们使用专门的高通量工具评估了供水对细胞壁可降解性、细胞壁含量和结构以及木质化细胞类型分布的影响，这些工具可有效地表型化来自 11 个自交系的玉米节间，在田间试验中，这些自交系在三年的两个对照灌溉方案下进行了处理。总的来说，我们的结果清楚地表明，水分亏缺导致木质素含量和分布发生显著变化，同时木质素对 p-香豆酸的酰化作用减少，从而影响细胞壁的可降解性。此外，我们还观察到，对水分亏缺的反应在被检查的系之间有所不同，这突出了植物育种的生化和组织学目标性状。
