Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan 4913815739, Iran.
Research Institute for Nanotechnology and Advanced Materials, Isfahan University of Technology, Isfahan, Iran.
Int J Biol Macromol. 2019 Oct 15;139:773-784. doi: 10.1016/j.ijbiomac.2019.07.182. Epub 2019 Jul 27.
Various concentrations of β-carotene (2.5-20% w/w dry mucilage weight) were loaded within Cydonia oblonga mucilage (COM) which further processed through electrohydrodynamic processing (EHP) to attain BC-Loaded nanostructures of high thermochemical stability. The BC loaded COM systems were characterized in terms of droplet size, rheological properties, surface tension, and electrical conductivity and their subsequent impacts on the morphology and physicochemical attributes of the produced nanostructures were studied. Increasing the β-carotene content, increased the viscosity and droplet size of colloidal systems but diminished their conductivity and surface tension. A transition from monomodal to bimodal size distribution was also observed at higher levels of β-carotene incorporation. In the case of EHP-produced nanostructures, scanning electron microscopy observations revealed a transition from nanoparticle to nanofiber by increasing the BC content from 2.5 to 20%. At lower concentrations of BC (2.5 and 5%) electrospraying was the dominant phenomenon that resulted in producing homogenous nanoparticles while the beaded fibers and nanofiber structures were obtained at 10 and 20% concentrations of BC, respectively. Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and encapsulation efficiency of the produced nanostructures revealed successful encapsulation of BC into the COM polymer structure through EHP. The results of these assessments showed that the nano-particles comprising BC had more homogenous structure and higher encapsulated BC compared to the BC-loaded nano-fibers. The results of X-ray diffraction (XRD) and differential scanning calorimetry (DSC) measurements revealed amorphous structure of the produced nanostructures. Thermogravimetric assessments corroborated the higher thermal stability of BC loaded nanostructures compared with the free BC. These results provided novel information on the ability of COM in encapsulating bioactive agents through electrohydrodynamic processing.
不同浓度的β-胡萝卜素（2.5-20%w/w 干胶重量）被负载到榅桲胶（COM）中，然后通过静电纺丝处理（EHP）进一步加工，以获得具有高热化学稳定性的 BC 负载纳米结构。通过测定液滴尺寸、流变性能、表面张力和电导率对负载 COM 系统进行了表征，并研究了其对所产生纳米结构的形态和物理化学性质的后续影响。随着β-胡萝卜素含量的增加，胶体系统的粘度和液滴尺寸增加，但电导率和表面张力降低。在较高的β-胡萝卜素掺入水平下，也观察到从单峰分布到双峰分布的转变。在 EHP 生产的纳米结构中，扫描电子显微镜观察表明，随着 BC 含量从 2.5%增加到 20%，从纳米颗粒到纳米纤维的转变。在 BC 浓度较低（2.5%和 5%）的情况下，电喷射是主要现象，导致产生均匀的纳米颗粒，而在 BC 浓度为 10%和 20%时，分别得到珠状纤维和纳米纤维结构。衰减全反射-傅里叶变换红外光谱（ATR-FTIR）和所产生的纳米结构的包封效率表明，通过 EHP 将 BC 成功包封到 COM 聚合物结构中。这些评估的结果表明，与负载 BC 的纳米纤维相比，包含 BC 的纳米颗粒具有更均匀的结构和更高的包封 BC。X 射线衍射（XRD）和差示扫描量热法（DSC）测量的结果表明所产生的纳米结构为非晶结构。热重评估证实了负载 BC 的纳米结构与游离 BC 相比具有更高的热稳定性。这些结果提供了关于 COM 通过静电纺丝处理包封生物活性物质的能力的新信息。
