Department of Pharmaceutics and Biopharmaceutics, University of Marburg, Robert-Koch-Str. 4, 35037 Marburg, Germany.
Department of Pharmacy, Biopharmaceutics and Pharmaceutical Technology, Saarland University, Campus C4 1, 66123 Saarbrücken, Germany.
Eur J Pharm Biopharm. 2019 Sep;142:531-539. doi: 10.1016/j.ejpb.2019.07.023. Epub 2019 Jul 27.
Antimicrobial resistance is one of the most serious problems that researchers of multiple disciplines are working on. The number of new antibiotics and their targeted structures have continuously decreased emphasizing the demand of alternative therapy for bacterial infections. Photodynamic therapy is such a promising strategy that has been proven to be effective against a wide range of bacterial strains. In this study, an inhalable nanoformulation for photodynamic therapy against respiratory infections was developed in the form of nano-in-microparticles consisting of curcumin nanoparticles embedded in a mannitol matrix. The produced nano-in-microparticles exhibited suitable aerodynamic properties with a mass median aerodynamic diameter of 2.88 ± 0.13 µm and a high fine particle fraction of 60.99 ± 9.50%. They could be readily redispersed in an aqueous medium producing the original nanoparticles without any substantial changes in their properties. This was confirmed using dynamic light scattering and electron microscopy. Furthermore, the redispersed nanoparticles showed an efficient antibacterial photoactivity causing 99.99992% (6.1log) and 97.75% (1.6log) reduction in the viability of Staphylococcus saprophyticus subsp. bovis and Escherichia coli DH5 alpha respectively. Based on these findings, it can be concluded that nano-in-microparticles represent promising drug delivery systems for antimicrobial photodynamic therapy.
抗菌耐药性是多个学科的研究人员正在努力解决的最严重问题之一。新抗生素的数量及其靶向结构不断减少，这强调了对抗细菌感染的替代疗法的需求。光动力疗法就是这样一种很有前途的策略，已被证明对多种细菌菌株有效。在这项研究中，以纳米-微颗粒的形式开发了一种用于呼吸道感染的光动力疗法的可吸入型纳米制剂，该制剂由嵌入甘露醇基质中的姜黄素纳米颗粒组成。所制备的纳米-微颗粒表现出合适的空气动力学特性，质量中值空气动力学直径为 2.88 ± 0.13 µm，细颗粒分数高达 60.99 ± 9.50%。它们可以很容易地在水介质中再分散，形成原始纳米颗粒，而其性质没有任何实质性变化。这一点通过动态光散射和电子显微镜得到了证实。此外，再分散的纳米颗粒显示出有效的抗菌光活性，分别使腐生葡萄球菌亚种和大肠杆菌 DH5 alpha 的活力降低了 99.99992%（6.1log）和 97.75%（1.6log）。基于这些发现，可以得出结论，纳米-微颗粒代表了用于抗菌光动力疗法的有前途的药物递送系统。
