Department of Environmental Engineering, Peking University, Beijing, 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing, 100871, China.
Department of Environmental Engineering, Peking University, Beijing, 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing, 100871, China.
Water Res. 2019 Oct 15;163:114872. doi: 10.1016/j.watres.2019.114872. Epub 2019 Jul 17.
One stage partial nitritation-anammox (PN-A) process has attracted more and more attention due to the low investment cost but the instability in treating low strength wastewater. In this study, for producing a novel composite carrier that could provide high ammonia microenvironment in low strength wastewater, the zeolites and floating materials were combined in the spherical shell and distributed evenly by the spherical polyhedron. And a moving bed biofilm reactor (MBBR) with the composite carriers and ordinary carriers without zeolites as control group was operated for nearly 120 days. The PN-A process were realized in 53 days, and the total nitrogen removal efficiency reached around 85% at influent ammonium concentration of 50 mg/L finally. Analysis of 16S rRNA gene sequencing revealed that the composite carriers showed significant promotion on the proliferation of ammonium oxidizing bacteria (AOB) and enrichment of anaerobic ammonium oxidizing bacteria (AnAOB), accounting for 19.14% and 41.65% on the surface, respectively. Moreover, the existence of relative higher abundance of ammonia on the composite carrier surface was validated by the metabolite biomarker of glutamate and especially spermidine. The metabolomics analysis and 16S rRNA function prediction showed that the protein synthesis pathway was obviously upregulated on the composite carriers surface compared with that on the ordinary carriers surface. The higher abundance of glutamate and putrescine indicated that the composite carrier could stimulate the metabolism and growth of bacteria. The present study provided a functional carrier to realize the transformation of activated sludge system into PN-A system treating low strength wastewater, which is significant to the application of the process in mainstream.
单级部分亚硝化-厌氧氨氧化（PN-A）工艺由于投资成本低而受到越来越多的关注，但在处理低浓度废水时不稳定。在这项研究中，为了生产一种新型的复合载体，能够在低浓度废水中提供高氨微环境，沸石和浮体被结合在球形壳中，并通过球形多面体均匀分布。然后，以含有沸石的复合载体和不含沸石的普通载体为对照组，运行了一个移动床生物膜反应器（MBBR）近 120 天。在 53 天内实现了 PN-A 工艺，最终在进水氨氮浓度为 50mg/L 时，总氮去除效率达到了 85%左右。16S rRNA 基因测序分析表明，复合载体对氨氧化细菌（AOB）的增殖和厌氧氨氧化细菌（AnAOB）的富集有显著的促进作用，分别占表面的 19.14%和 41.65%。此外，通过谷氨酸和特别是亚精胺等代谢物生物标志物，验证了复合载体表面存在相对较高丰度的氨。代谢组学分析和 16S rRNA 功能预测表明，与普通载体表面相比，复合载体表面的蛋白质合成途径明显上调。谷氨酸和腐胺含量较高表明，复合载体可以刺激细菌的代谢和生长。本研究提供了一种功能载体，实现了活性污泥系统向 PN-A 系统的转化，用于处理低浓度废水，这对该工艺在主流中的应用具有重要意义。
