Department of Surgery, Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
Int J Mol Sci. 2019 Jul 29;20(15):3704. doi: 10.3390/ijms20153704.
Mouse hind limb ischemia is the most common used preclinical model for peripheral arterial disease and critical limb ischemia. This model is used to investigate the mechanisms of neovascularization and to develop new therapeutic agents. The literature shows many variations in the model, including the method of occlusion, the number of occlusions, and the position at which the occlusions are made to induce hind limb ischemia. Furthermore, predefined end points and the histopathological and radiological analysis vary. These differences hamper the correlation of results between different studies. In this review, variations in surgical methods of inducing hind limb ischemia in mice are described, and the consequences of these variations on perfusion restoration and vascular remodeling are discussed. This study aims at providing the reader with a comprehensive overview of the methods so far described, and proposing uniformity in research of hind limb ischemia in a mouse model.
鼠标后肢缺血是最常用的用于外周动脉疾病和严重肢体缺血的临床前模型。该模型用于研究血管新生的机制和开发新的治疗药物。文献显示该模型有许多变化，包括闭塞方法、闭塞次数和进行闭塞的位置，以诱导后肢缺血。此外，预设的终点和组织病理学及影像学分析也有所不同。这些差异阻碍了不同研究结果之间的相关性。在这篇综述中，描述了在小鼠中诱导后肢缺血的手术方法的变化，并讨论了这些变化对灌注恢复和血管重塑的影响。本研究旨在为读者提供迄今为止所描述方法的全面概述，并提出在小鼠后肢缺血模型的研究中实现一致性。
