Pandey Sony, Hickey Dawn U, Drum Marti, Millis Darryl L, Cekanova Maria
Department of Small Animal Clinical Siences, College of Veterinary Medicine, The University of Tennessee, Knoxville, 37996, TN, USA.
UT-ORNL Graduate School of Genome Science and Technology, The University of Tennessee, Knoxville, 37996, TN, USA.
BMC Vet Res. 2019 Jul 30;15(1):269. doi: 10.1186/s12917-019-2010-x.
Reported efficacy of platelet-rich plasma (PRP) in regenerative medicine is contradictory. We validated the effects of PRP on proliferation of canine bone marrow-derived multipotent mesenchymal stromal cells (K9BMMSCs) in vitro. PRP was extracted from blood of six dogs with osteoarthritis. K9BMMSCs were established from bone marrow and characterized for CD90 and CD19 expression by immunocytochemistry. Effects of PRP concentrations on viability of matching autologous K9BMMSCs were validated using MTS assay.
Positive CD90 and negative CD19 expression confirmed MSC origin. PRP at 40% volume/volume concentration increased, while PRP at 80 and 100% v/v concentrations suppressed viability of tested K9BMMSCs.
PRP concentration plays an important role in K9BMMSCs viability, which could affect tissue repairs in vivo.
富血小板血浆（PRP）在再生医学中的疗效报道相互矛盾。我们在体外验证了PRP对犬骨髓来源的多能间充质基质细胞（K9BMMSCs）增殖的影响。PRP从六只患骨关节炎犬的血液中提取。从骨髓中分离出K9BMMSCs，并通过免疫细胞化学鉴定其CD90和CD19表达。使用MTS测定法验证PRP浓度对匹配的自体K9BMMSCs活力的影响。
CD90阳性和CD19阴性表达证实了间充质基质细胞的来源。40%体积/体积浓度的PRP可提高K9BMMSCs活力，而80%和100%体积/体积浓度的PRP则抑制受试K9BMMSCs的活力。
PRP浓度在K9BMMSCs活力中起重要作用，这可能影响体内组织修复。
