Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
Program in Public Health, University of California Irvine, Irvine, California, USA.
Parasit Vectors. 2019 Jul 30;12(1):382. doi: 10.1186/s13071-019-3646-x.
The Asian tiger mosquito, Aedes albopictus, an increasingly relevant arboviral vector, has spread worldwide. However, currently available tools are limited in terms of effective monitoring of vector populations and accurate determination of the extent of viral transmission, especially before and during outbreaks. Therefore, it is essential to develop novel monitoring and surveillance tools, particularly those that target adult mosquitoes and enhance the trapping efficiency for Ae. albopictus.
A variety of human body odorants associated with different types of mosquito olfactory receptors were selected, and their attractiveness to Ae. albopictus was tested by a four-arm olfactometer. The optimal compatibility and proportion of the odorants, Mix-5, was observed via orthogonal design analyses. The attractiveness of Mix-5 to Ae. albopictus in the laboratory was assessed using Mosq-ovitraps and Electric Mosquito Killers. In the field, the effectiveness of generic BG-Lure, Mix-5 and a control treatment was compared with a baited Biogents Sentinel trap (BGS-trap) using a Latin square design.
In the olfactometer experiments, the attractiveness of the selected candidate compounds at varying dilutions was poor when the individual compounds were used alone. The optimal combination, Mix-5, was generated based on orthogonal design analyses. In the laboratory, the average numbers of female Ae. albopictus mosquitoes attracted by the synthetic odorant blend Mix-5 were 27.00 and 27.50, compared with 12.00 and 14.83 for the control, when using Mosq-ovitraps and Electric Mosquito Killers, respectively. In the field, the average number of Ae. albopictus female mosquitoes trapped by Mix-5 was 9.67 females/trap, whereas the average numbers for BG-Lure and the control were 7.78 and 4.47, respectively. The lure also played an important role in attracting Culex quinquefasciatus mosquitoes, and the average numbers of Cx. quinquefasciatus female mosquitoes attracted by Mix-5, BG-Lure and the control were 18.78, 25.11 and 12.22, respectively.
A human odor-based bait blend was developed and exhibited enhanced effectiveness at attracting Ae. albopictus This blend can be used to monitor and trap dengue vector mosquitoes in Chinese cities.
亚洲虎蚊（Aedes albopictus）作为一种日益重要的虫媒病毒载体，已在全球范围内传播。然而，目前可用的工具在有效监测病媒种群和准确确定病毒传播程度方面存在局限性，尤其是在疫情爆发之前和期间。因此，开发新型监测和监测工具至关重要，特别是那些针对成年蚊子并提高诱捕亚洲虎蚊效率的工具。
选择了与不同类型蚊子嗅觉受体相关的各种人体气味，并通过四臂嗅觉仪测试了它们对亚洲虎蚊的吸引力。通过正交设计分析观察到气味混合物 Mix-5 的最佳相容性和比例。通过 Mosq-诱捕器和电蚊拍评估了 Mix-5 对实验室中亚洲虎蚊的吸引力。在野外，使用带有诱饵的 Biogents 哨兵诱捕器（BGS-诱捕器），通过拉丁方设计比较了通用 BG-Lure、Mix-5 和对照处理的效果。
在嗅觉仪实验中，当单独使用时，所选候选化合物在不同稀释度下的吸引力很差。根据正交设计分析生成了最佳组合 Mix-5。在实验室中，与对照相比，合成气味混合物 Mix-5 吸引的雌性亚洲虎蚊的平均数量分别为 Mosq-诱捕器和电蚊拍的 27.00 和 27.50，分别为 12.00 和 14.83。在野外，Mix-5 诱捕的雌性亚洲虎蚊平均数量为 9.67 只/诱捕器，而 BG-Lure 和对照的平均数量分别为 7.78 和 4.47。该诱捕器在吸引库蚊方面也发挥了重要作用，Mix-5、BG-Lure 和对照吸引的雌性库蚊平均数量分别为 18.78、25.11 和 12.22。
开发了一种基于人体气味的诱饵混合物，并显示出对吸引亚洲虎蚊的增强效果。这种混合物可用于监测和诱捕中国城市的登革热病媒蚊子。
