[丝裂霉素C给药后肿瘤细胞蛋白质斑点二维凝胶电泳图谱的变化]
[Changes in two-dimensional gel electrophoretic patterns of cellular protein spots in tumors after administration of mitomycin C].
作者信息
Takahashi T
机构信息
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
出版信息
Nihon Geka Gakkai Zasshi. 1988 Apr;89(4):494-507.
The changes of cellular protein spots in human stomach carcinomas (Exp 4, H-111) and colon carcinomas (SW403, SW480) implanted into nude mice were examined after administration of MMC. The results were as follows. 1) Exp4 histologically showed pleomorphism after treatment by MMC. In other tumors no changes in histological features were shown. 2) The most remarkable decreases of p protein spots in Exp4, and moderate decreases of protein spots in SW403 and SW480 were admitted, contrasting to no changes in the pattern of protein spots in H-111. 3) The decreases of protein spots in the tumors were related to the sensitivities to MMC. 4) Regrowth tumors in Exp4 which were survived after administration of MMC revealed similar protein spots patterns and histological features to those in control tumors. 5) The changes in protein spots in the liver and kidney derived from normal mice were also examined. In these, protein spots patterns showed no changes after administration of MMC. These results suggest that the cellular protein sports were decreased in accordance with effect of MMC in the tumors.
在给予丝裂霉素（MMC）后，检测了接种于裸鼠体内的人胃癌（实验4，H - 111）和结肠癌（SW403、SW480）细胞蛋白斑点的变化。结果如下：1）实验4经MMC处理后，组织学上显示出多形性。其他肿瘤的组织学特征未显示出变化。2）实验4中p蛋白斑点显著减少，SW403和SW480中蛋白斑点中度减少，与之形成对比的是，H - 111中蛋白斑点模式无变化。3）肿瘤中蛋白斑点的减少与对MMC的敏感性相关。4）实验4中在给予MMC后存活下来的再生长肿瘤显示出与对照肿瘤相似的蛋白斑点模式和组织学特征。5）还检测了正常小鼠肝脏和肾脏中蛋白斑点的变化。在这些组织中，给予MMC后蛋白斑点模式未显示出变化。这些结果表明，肿瘤细胞中的蛋白斑点随MMC的作用而减少。
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