Kondo T, Imaizumi M, Taguchi T, Abe O, Hattori T, Wakui A, Kitano M
Gan To Kagaku Ryoho. 1987 Mar;14(3 Pt 1):680-6.
Human tumors transplanted into nude BALB/c-nu mice have been used to test the sensitivity of the tumors to various anticancer agents. Three cancer cell lines from the stomach and one from the colon were transplanted into nude mice to establish a standard assay method for selection of effective drugs on individual tumors using the criteria of the Japanese Association of Sensitivity Determination for Carcinostatic Agents. From the LD values of anticancer drugs in nude mice, the appropriate doses of drugs were 6 mg/kg of MMC X 1(i.p.), 50 mg/kg of 5-FU q4d X 3 (i.p.), 120 mg/kg of CPA (i.p.), 30 mg/kg of ACNU (i.p.) 8 mg/kg of CDDP (i.p.) and 8 mg/kg of ADM (i.v.). At 3 weeks after initial treatment, the inhibition rate (IR) of the tumor was calculated from the formula IR = (1-T/C) X 100%, where T is the mean tumor weight of the treated group and C is the mean weight of the untreated group at that time. The tumors respond well to the anticancer agents when IR is more than 58%.
将人类肿瘤移植到裸BALB/c-nu小鼠体内，用于测试肿瘤对各种抗癌药物的敏感性。将三种胃癌细胞系和一种结肠癌细胞系移植到裸鼠体内，以建立一种标准检测方法，按照日本抗癌剂敏感性测定协会的标准，针对个体肿瘤选择有效药物。根据抗癌药物在裸鼠体内的半数致死量（LD）值，合适的药物剂量为：丝裂霉素（MMC）6mg/kg×1次（腹腔注射）、氟尿嘧啶（5-FU）50mg/kg，每4天1次，共3次（腹腔注射）、环磷酰胺（CPA）120mg/kg（腹腔注射）、阿糖胞苷（ACNU）30mg/kg（腹腔注射）、顺铂（CDDP）8mg/kg（腹腔注射）和阿霉素（ADM）8mg/kg（静脉注射）。初始治疗3周后，根据公式IR = (1 - T/C)×100%计算肿瘤抑制率（IR），其中T为治疗组的平均肿瘤重量，C为当时未治疗组的平均重量。当IR超过58%时，肿瘤对抗癌药物反应良好。
