盘基网柄菌的弹性模量受机械转导影响。
Elastic modulus of Dictyostelium is affected by mechanotransduction.
作者信息
Wu Yan, Cooper Kate M
机构信息
Department of Engineering Physics, University of Wisconsin-Platteville, 1 University Plaza, Platteville, WI, 53818, USA.
Department of Biology, Loras College, 1450 Alta Vista Street, Dubuque, IA, 52001, USA.
出版信息
J Biol Phys. 2019 Sep;45(3):293-305. doi: 10.1007/s10867-019-09529-1. Epub 2019 Jul 30.
The stiffness of adherent mammalian cells is regulated by the elasticity of substrates due to mechanotransduction via integrin-based focal adhesions. Dictyostelium discoideum is an ameboid protozoan model organism that does not carry genes for classical integrin and can adhere to substrates without forming focal adhesions. It also has a life cycle that naturally includes both single-cellular and multicellular life forms. In this article, we report the measurements of the elastic modulus of single cells on varied substrate stiffnesses and the elastic modulus of the multicellular "slug" using atomic force microscopy (AFM) as a microindenter/force transducer. The results show that the elastic modulus of the Dictyostelium cell is regulated by the stiffness of the substrate and its surrounding cells, which is similar to the mechanotransduction behavior of mammalian cells.
由于通过基于整合素的粘着斑进行机械转导，贴壁哺乳动物细胞的硬度受底物弹性的调节。盘基网柄菌是一种变形虫原生动物模式生物，它不携带经典整合素基因，并且可以在不形成粘着斑的情况下附着于底物。它还具有一个自然包含单细胞和多细胞生命形式的生命周期。在本文中，我们报告了使用原子力显微镜（AFM）作为微压头/力传感器，测量不同底物硬度下单细胞的弹性模量以及多细胞“蛞蝓体”的弹性模量。结果表明，盘基网柄菌细胞的弹性模量受底物及其周围细胞硬度的调节，这与哺乳动物细胞的机械转导行为相似。
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