Department of Respiratory Medicine, The First People's Hospital of Yunnan Province, Kunming, China.
Eur Rev Med Pharmacol Sci. 2019 Jul;23(14):6211-6216. doi: 10.26355/eurrev_201907_18438.
To explore the role of phospholipase C epsilon 1 (PLCE1) in regulating cell apoptosis of non-small cell lung cancer (NSCLC) and its underlying mechanism.
The mRNA and protein levels of PLCE1 in NSCLC tissues, adjacent normal tissues and NSCLC cell lines (A549 and H1299) were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot, respectively. Methylation status in the promoter region of PTEN in NSCLC cells was accessed using the relative commercial kit. Cell apoptosis after transfection of PLCE1 siRNA in NSCLC cells was detected by flow cytometry. Protein expressions of apoptosis-related genes in NSCLC cells after altering PLCE1 expression were detected by Western blot.
PLCE1 was highly expressed in NSCLC tissues and cell lines than that of controls. PLCE1 knockdown promoted PTEN expression and inhibited methylation in H1299 cells. Transfection of PLCE1 siRNA in NSCLC cells remarkably induced cell apoptosis.
PLCE1 inhibits cell apoptosis of NSCLC by promoting PTEN methylation.
探讨磷酯酶 C ɛ 1（PLCE1）在调控非小细胞肺癌（NSCLC）细胞凋亡中的作用及其机制。
采用实时定量聚合酶链反应（qRT-PCR）和 Western blot 检测 NSCLC 组织、癌旁正常组织和 NSCLC 细胞系（A549 和 H1299）中 PLCE1 的 mRNA 和蛋白水平，使用相关商业试剂盒检测 NSCLC 细胞中 PTEN 启动子区的甲基化状态。通过转染 PLCE1 siRNA 检测 NSCLC 细胞中细胞凋亡，通过 Western blot 检测改变 PLCE1 表达后 NSCLC 细胞中凋亡相关基因的蛋白表达。
PLCE1 在 NSCLC 组织和细胞系中的表达明显高于对照组。PLCE1 敲低促进了 H1299 细胞中 PTEN 的表达并抑制了其甲基化。转染 NSCLC 细胞中的 PLCE1 siRNA 可显著诱导细胞凋亡。
PLCE1 通过促进 PTEN 甲基化抑制 NSCLC 细胞凋亡。
