Department of Cardiothoracic Surgery, Nanhai People's Hospital, the Nanfang Medical University Affiliated Nanhai Hospital, Foshan, China.
Cell Biochem Funct. 2014 Apr;32(3):294-8. doi: 10.1002/cbf.3015. Epub 2013 Dec 20.
The pathogenesis of lung cancer is to be further investigated. Recent reports indicate that phospholipase C ε-1 (PLCE1) is a critical molecule involved in tumour growth. This study aims to investigate the role of PLCE1 in the regulation of apoptosis in lung cancer cells. In this study, the surgically removed non-small-cell lung cancer (NSCLC) tissue was collected from 36 patients. Single NSCLC cells were prepared from the tissue, in which immune cells of CD3(+) , CD11c(+) , CD19(+) , CD68(+) and CD14(+) were eliminated by magnetic cell sorting. The expression of PLCE1 and p53 was assessed by quantitative real-time polymerase chain reaction and Western blotting. Apoptosis of NSCLC cells was analysed by flow cytometry. The results showed that, in cultured NSCLC cells, high levels of PLCE1 and low levels p53 were detected; the two molecules showed a negative correlation (p < 0.01). The addition of anti-PLCE1 antibody increased the expression of p53 in NSCLC cells, which increased the frequency of apoptotic NSCLC cells. We conclude that NSCLC cells express high levels of PLCE1, which suppresses the expression of p53 in NSCLC cells. PLCE1 can be a therapeutic target of NSCLC.
肺癌的发病机制有待进一步研究。最近的报告表明，磷脂酶 C ε-1（PLCE1）是参与肿瘤生长的关键分子。本研究旨在探讨 PLCE1 在肺癌细胞凋亡调控中的作用。在这项研究中，从 36 名患者中采集了手术切除的非小细胞肺癌（NSCLC）组织。从组织中制备了单个 NSCLC 细胞，通过磁细胞分选消除了 CD3(+)、CD11c(+)、CD19(+)、CD68(+)和 CD14(+)的免疫细胞。通过实时定量聚合酶链反应和 Western blot 评估 PLCE1 和 p53 的表达。通过流式细胞术分析 NSCLC 细胞的凋亡。结果表明，在培养的 NSCLC 细胞中，检测到高水平的 PLCE1 和低水平的 p53；这两种分子呈负相关（p<0.01）。添加抗 PLCE1 抗体增加了 NSCLC 细胞中 p53 的表达，从而增加了凋亡的 NSCLC 细胞的频率。我们得出结论，NSCLC 细胞表达高水平的 PLCE1，抑制 NSCLC 细胞中 p53 的表达。PLCE1 可以成为 NSCLC 的治疗靶点。
